cFluor^® B532 Anti-Mouse CD8a (53-6.7)

The rat monoclonal antibody clone 53-6.7 binds to the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha) but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase Lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by viruses, bacteria, or parasites. It can be used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells and thymocytes and on specific cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. PRODUCT DETAILSClone: 53-6.7Specificity: CD8aReactivity: MouseFormat: cFluor® B532Isotype: Rat IgG2a, kTested Dilution: 5 µl/TestApplication: Flow CytometryStorage: 2-8°C protected from light. Do not freezeFormulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)Data Sheets: TDS | SDS RECOMMENDED USAGEEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. Please briefly centrifuge the reagent vial before use. Use appropriate personal protective equipment per the product safety data sheet when using this product. REFERENCES van Ewijk W. et al. 1981. J. Immunol.127:2594-2604. Ledbetter JA, 1980. J Exp Med.152:280-295. For research use only. Not intended for use in diagnostic procedures. cFluor® B532 is equivalent to CF®503R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.