T7 RNA Transcription Enzyme Mix (low dsRNA) _ 10632ES

This product is a phage T7 RNA polymerase derived from recombinant expression in E. coli. It is an optimized mutant of wild-type T7, formulated as a mixture of T7 RNA polymerase variants that significantly reduce dsRNA content. Using double-stranded DNA containing the T7 promoter sequence as a template and NTPs as substrates, it synthesizes RNA complementary to the strand downstream of the promoter. Double-stranded linear blunt-ended or 5'-overhanging DNA can serve as substrate templates for T7 RNA polymerase; therefore, linearized plasmids and PCR products can both be used as templates for in vitro RNA synthesis. Modified nucleotides can be added to the substrates during transcription to prepare biotin- or dye-labeled RNA. Specifications Catalog No. 10632ES20 / 10632ES60 / 10632ES70 / 10632ES80 Size 20 μL / 100 μL / 200 μL / 1 mL Component Information Component 10632ES20 10632ES60 10632ES70 10632ES80 T7 RNA Polymerase Mix (low dsRNA) 20 μL 100 μL 200 μL 1 mL Storage Store at -25°C to -15°C. Valid for 1 year. Documents: Safety Data Sheet 10632_MSDS_HB260814 Manuals 10632_Manual_HB20260814