1X dsDNA HS Assay Kit

Description Laboratories measuring low concentrations of double-stranded DNA may find that UV absorbance at 260 nm is affected by other substances in the sample. Preparing a working solution before every assay also adds a handling step. The Biofargo 1× dsDNA HS Assay Kit provides a ready-to-use, fluorescence-based method for high-sensitivity dsDNA quantification. The supplied 1× dsDNA HS Working Solution requires no dilution. Add the sample, incubate for 2 minutes at room temperature protected from light, and measure the fluorescence. The assay is highly selective for dsDNA over RNA. It accepts 1–20 µL of sample in a final assay volume of 200 µL. The current product details state an initial dsDNA concentration range of 10 pg/µL–100 ng/µL, depending on sample volume, and a total assay range of 0.2–100 ng. The kit supports Qubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers. It can also be used with a fluorescence microplate reader at 485 nm excitation and 530 nm emission. When protected from light, the fluorescent signal remains stable for up to 3 hours after incubation. For Research Use Only. Not for use in diagnostic or therapeutic procedures. Specifications Parameter Specification Product Name Biofargo 1× dsDNA HS Assay Kit Assay Type High Sensitivity dsDNA Assay Target Double-stranded DNA (dsDNA) Detection Method Fluorescence Formulation Ready-to-use 1× dsDNA HS Working Solution Initial Sample Concentration 10 pg/µL–100 ng/µL, depending on sample volume Total Assay Range 0.2–100 ng Sample Volume 1–20 µL Final Assay Volume 200 µL per tube or well Assay Temperature Room temperature, 20–25°C Incubation 2 minutes at room temperature, protected from light Signal Stability Up to 3 hours after incubation when protected from light Compatible Fluorometers Qubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers Microplate Reader Compatibility 485 nm excitation and 530 nm emission Recommended Microplate Black-wall, clear-bottom 96-well microplate Shipping Condition Ship on ice Storage 2–8°C, protected from light Intended Use Research Use Only Features Ready-to-use formulation: The supplied 1× dsDNA HS Working Solution can be used without dilution. High-sensitivity dsDNA quantification: The current product details state a total assay range of 0.2–100 ng. Selective dsDNA measurement: The assay is highly selective for double-stranded DNA over RNA. Flexible sample input: Each assay accepts 1–20 µL of sample in a final volume of 200 µL. Short incubation: Incubate for 2 minutes at room temperature while protected from light. Stable reading window: The fluorescent signal remains stable for up to 3 hours when protected from light. Multiple instrument formats: The kit supports the specified Qubit fluorometers and a fluorescence microplate reader. Contaminant tolerance: DNA standards were evaluated with BSA, sodium acetate, ammonium acetate, sodium chloride, magnesium chloride, phenol, chloroform, ethanol, SDS, Triton X-100, ssDNA, polyethylene glycol, RNA, and dNTPs. In the supplied results, “OK” usually represents less than 10% perturbation at the indicated final assay concentration. Applications High-Sensitivity dsDNA Quantification with a Qubit Fluorometer: Equilibrate the kit components to room temperature. Use thin-wall, clear 0.5 mL PCR tubes and label their lids rather than their sides. Prepare each standard with 190 µL of 1× dsDNA HS Working Solution and 10 µL of QS0 or QS1. Prepare each sample with 180–199 µL of Working Solution and 1–20 µL of sample, for a final volume of 200 µL. Use a 2 µL pipette when adding 1–2 µL of sample. Vortex each tube for 3–5 seconds, then incubate for 2 minutes at room temperature protected from light. On a Qubit 4 Fluorometer, select dsDNA, then 1× dsDNA High Sensitivity. Read QS0 followed by QS1, select the sample volume and output units, and read the samples. Avoid warming the tubes by holding them before measurement. Do not use calibration curves generated with other kits. High-Sensitivity dsDNA Quantification with a Fluorescence Microplate Reader: Equilibrate the kit components to room temperature and mix thoroughly. Use a black-wall, clear-bottom 96-well microplate. Add 190 µL of Working Solution to each standard well and 180–199 µL to each sample well. Serially dilute QS1 to prepare standards from 0 to 10 ng/µL, then add 10 µL of each standard to its designated well. Add 1–20 µL of each sample so every well contains exactly 200 µL. Vortex the plate for 10–15 seconds and incubate for 2 minutes at room temperature protected from light. Read at 485 nm excitation and 530 nm emission. Generate a standard curve to calculate unknown concentrations. If a sample exceeds the linear range, dilute it and repeat the assay. Kit Components Component Name FS-T1005-S — 100 Reactions FS-T1005 — 1,200 Reactions 1× dsDNA HS Working Solution 20 mL 250 mL dsDNA HS Standard QS0 (0 ng/µL) 100 µL 5 mL dsDNA HS Standard QS1 (10 ng/µL) 100 µL 5 mL Storage & Shipping Store at 2–8°C, protected from light. Ship on ice. Frequently Asked Questions (FAQs) What is the Biofargo 1× dsDNA HS Assay Kit? It is a ready-to-use, high-sensitivity fluorescence assay for quantifying double-stranded DNA. The kit includes a premixed 1× dsDNA HS Working Solution and two dsDNA standards. Does the 1× dsDNA HS Working Solution require dilution? No. The supplied solution is ready to use and does not require preparation or dilution before adding standards or samples. What is the assay range? The current product details state a total assay range of 0.2–100 ng and an initial dsDNA concentration range of 10 pg/µL–100 ng/µL, depending on sample volume. Is the kit compatible with Qubit fluorometers? Yes. The protocol supports Qubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers. On a Qubit 4 Fluorometer, select dsDNA and then 1× dsDNA High Sensitivity. Can the kit be used with a fluorescence microplate reader? Yes. Use a black-wall, clear-bottom 96-well microplate and set the reader to 485 nm excitation and 530 nm emission. How much sample is required for each assay? Use 1–20 µL of sample in a final assay volume of 200 µL. When adding 1–2 µL of sample, use a 2 µL pipette. How long should the assay be incubated? Incubate for 2 minutes at room temperature protected from light. The signal remains stable for up to 3 hours when protected from light. What catalog numbers and kit sizes are available? FS-T1005-S is listed as 100 reactions, and FS-T1005 is listed as 1,200 reactions. How should the kit be shipped and stored? Ship on ice. Store all components at 2–8°C protected from light. Documents Product User Manual