HiActi™ Recombinant Human FGF-6 Protein, His Tag _ 91351ES

Fibroblast growth factor-6 (FGF-6) is a cytokine belonging to the heparin-binding members of the FGF family. Members of this family exhibit broad mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF-6 binds to and signals through the FGF receptors FGFR1 and FGFR4. It is also essential for normal muscle regeneration. In vivo, high concentrations of recombinant FGF-6 upregulate FGFR1 and downregulate FGFR4, respectively, as FGFR1 promotes proliferation while FGFR4 promotes muscle differentiation. Transfection of the FGF6 gene into mammalian cells has been shown to possess oncogenic transformation activity. Although FGF-6 serves a protective function against mechanical muscle injury, it is overexpressed in the developing heart and regulates osteoblasts and osteoclasts during bone remodeling. Specifications Cat.No. 91351ES10 / 91351ES50 / 91351ES60 / 91351ES76 Size 10 μg / 50 μg / 100 μg / 500 μg Components Name 91351ES10 91351ES50 91351ES60 91351ES76 HiActiTM Recombinant Human FGF-6 Protein, His Tag 10 μg 50 μg 100 μg 500 μg Performance parameters Synonyms fibroblast growth factor 6, HBGF-6, HST-2, FGF6 Species Human Expression Sequence Gly41-Ile208 Accession P10767 Tag C-His Expression System E.coli Molecular Weight 18-19 kDa(Reducing) Purity >95% as determined by SDS-PAGE and HPLC. Endotoxin <1.0 EU per 1 μg of the protein by the LAL method. Formulation PBS, pH7.4 Appearance Lyophilized Powder Biological activity Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The EC50 for this effect is typically less than 5 ng/mL. Reconstitution Instructions Centrifuge the tube before opening to collect the contents at the bottom. Reconstitute with ultra-pure water to a concentration of 0.1–0.5 mg/mL. The solution can be further diluted according to subsequent experimental requirements. Storage Store at -25 to -15°C. Valid for 1 year from receipt. After reconstitution, store at 2 to 8°C for up to 7 days, or at -85 to -65°C for up to 3 months. To avoid repeated freeze-thaw cycles, we recommend aliquoting the protein upon first use. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves, for your safety. Documents Safety Data Sheet 91351_MSDS_HB260723 Manuals: 91351_Manual_HB20260615