Defined Bioscience's HiDef® S8 is a complete, serum-free, defined formulation designed for the high-density, microcarrier-free suspension expansion of human pluripotent stem cells (PSCs) in stirred-tank bioreactors and other suspension culture systems. HiDef® S8 is currently provided as: The HiDef® S8 400X Supplement only (DMEM/F12 basal medium and optional surfactant NOT included), allowing users the flexibility to select their preferred basal medium and suspension workflow components. To better support small-scale experiments and pilot studies, the HiDef® S8 400X Supplement is offered in two vial sizes. Standard vials prepare 500 mL of complete medium, while miniature vials prepare 50 mL, enabling convenient preparation of smaller volumes without excess waste. See instructions below regarding preparation of each format. More Details HiDef® S8 is a unique formulation that enables high-yield, scalable suspension expansion of human pluripotent stem cells without the need for microcarriers. The medium supports efficient aggregate formation from single-cell or small aggregate inoculation, rapid biomass accumulation, and expansion to tens of millions of PSCs per milliliter while maintaining high viability, uniform aggregate morphology, stable size distribution, and expression of key pluripotency markers. Following suspension expansion, cells readily transition back to adherent culture while preserving normal morphology and pluripotent characteristics. HiDef® S8 contains a combination of essential nutrients and growth factors formulated specifically to maintain pluripotency while supporting robust suspension growth under agitation conditions. The addition of further components is not required, although optional surfactants such as polyvinyl alcohol (PVA), Pluronic® F-68, dextran sulfate, and others may be incorporated where desired for specific suspension workflows. HiDef® S8 400X Supplement has been verified for compatibility with standard formulations of DMEM/F12 from several manufacturers, including Corning® (#10-092), Gibco™ (#11330), and GenClone™ (#25-503). Optional surfactants including 0.1% (w/v) polyvinyl alcohol (PVA), 0.1% (w/v) Pluronic® F-68, dextran sulfate, and related additives may be used depending on the application and bioreactor configuration. This list is provided for reference and is not an exhaustive list of verified compatible components. Contact us at info@definedbioscience.com for additional compatibility information. Each lot of HiDef® S8 400X Supplement is used in combination with basal medium during performance qualification using multiple human PSC lines cultured under suspension conditions in both ambient and hypoxic environments.