Recombinant Human BMP-4 Protein, Active

Product Overview Product Name Recombinant Human BMP-4 Protein Target Symbol BMP4 Alternative Names Bone Morphogenetic Protein 4, Bone Morphogenetic Protein 2B, BMP4, BMP2B, BMP2B1, MCOPS6, OFC11, ZYME, DVR4, ZBMP4 Accession Number P12644.1 Expression System Mammalian cell line. Molecular Weight 13 kDa (monomer, predicted) Sequence Ser293-Arg408 Purity Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining Activity Determined by in-house activity assay Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4 Endotoxin Level Available Upon Request Shipping Shipped at ambient temperature. Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. Reconstitution Reconstitute at 100 ug/mL in sterile PBS. Target Function Bone Morphogenetic Protein 4 (BMP-4) plays roles across bone formation, embryonic development, organogenesis, cell differentiation and tissue repair. BMP-4 promotes the differentiation of mesenchymal stem cells into osteoblasts, as well as differentiation of neuronal precursors, cardiac progenitors, kidney progenitors, keratinocyte differentiation, odontoblast differentiation, adipocyte differentiation, and regulation of ovarian follicle development. Within the ectoderm and mesoderm, BMP-4 contributes to dorsal-ventral patterning of the developing neural tube, as well as additional patterning leading to development of lung, bone, kidney, eye, heart, muscle and limbs. In response to tissue damage, BMP-4 promotes migration of keratinocytes and angiogenesis. BMP-4 signaling occurs via binding to BMPR1A (CD292) and BMPR2, at which point BMPR2 transphosphorylates BMPR1A to induce the Smad signaling pathway. Tissue Specificity BMP-4 expression has been identified in ectoderm, mesoderm, endoderm, developing skeletal tissues such as cartilage and bone, the developing central nervous system, including the neural tube and the brain, heart, kidney, lung, skin, ovaries, testes, and tooth bud. Cellular Localization Secreted protein Involvement In Disease Dysregulation of BMP-4 is associated with osteoporosis, skeletal dysplasias, craniofacial abnormalities such as cleft lip and palate, pulmonary arterial hypertension, atherosclerosis, cardiac fibrosis, Alzheimer’s disease, Parkinson’s disease, insulin resistance, obesity, metabolic syndrome, polycystic kidney disease, renal fibrosis, diabetic nephropathy, rheumatoid arthritis, osteoarthritis, and various cancers due to BMP-4 enhanced cell proliferation, apoptosis, invasion, and metastasis of cancer cells.