SuperMethyl™ Max Bisulfite Conversion Kit _ 17328ES

The SuperMethyl™ Max Bisulfite Conversion Kit from Ellis Bio offers the exceptionally mild yet powerful bisulfite conversion for DNA methylation analysis. Featuring a newly engineered ultra-mild bisulfite conversion formulation and a magnetic bead-based DNA purification method, this kit simplifies the methyl conversion workflow and achieves efficient conversion with minimal DNA degradation. Complete bisulfite conversion and DNA purification can be accomplished in 3 hours or less, preserving DNA integrity even with ultra-low input samples, while consistently achieving 99.8% conversion efficiency. Its broad applicability makes it a valuable tool for both research and translational settings. Designed for compatibility and consistent performance in sensitive and/or low-input DNA samples, the SuperMethyl™ Max Bisulfite Conversion Kit delivers reliable, reproducible results, making it the preferred choice for precision-focused researchers. Features Ultra-Mild Conversion Chemistry:Delivers excellent DNA library yield and larger library insert sizes, preserving sample integrity. Low-Input Friendly:Efficiently processes as little as 100 pg of DNA, ideal for limited or precious samples. Exceptional Conversion Efficiency:Achieves 99.8% C-to-T conversion, ensuring unmatched accuracy in methylation analysis. Uniform GC Coverage:Provides more even GC representation, enhancing coverage across promoters and GC-rich regions. Simplified, Rapid Workflow:Up to 3× faster with 5× less hands-on time than enzyme-based methods. Convenient Bead-Based Purification Format:Streamlined purification for improved yield and ease of use. Automation liquid handler friendly. Component Component Volume/Ǫuantity Storage Kit Capacity 24 Tests Please see storage recommendations for individual components Conversion Reagent ◉ 2 mL 4 °C (recommended) Preparation Reagent ◉ 120 µL Room temperature Enhancer A ◉ 120 µL Room temperature Enhancer B ◉ 240 µL 4 °C (recommended) Binding Buffer 22 mL Room temperature Wash Buffer (concentrate)* 7 mL* Room temperature Desulphonation Buffer 6 mL Room temperature Elution Buffer 1 mL Room temperature Purification Beads ◉ 400 µL Room temperature * Wash Buffer requires the addition of 28 mL 100% ethanol (EtOH) before first use. Shipping Conditions No Cold Chain Required; Shipped at Room Temperature Precautions / Handling Instructions Upon receipt of the kit, store the Conversion Reagent and Enhancer B at 4°C. Workflow Figures 1. Figure 1 2. Figure 2 3. Figure 3 4.Versatile and robust methyl-seq performance Figure 4. Key performance metrics from whole-genome and targeted methylation profiling with the KAPA EvoPrep Kit and different conversion chemistries. Documents: Safety Data Sheet MSDS Manuals Manual Additional Resources: Data sheet cfDNA Data Brief gDNA Data Brief Microarray Data Brief