cFluor^® B532 Anti-Mouse CD3 (17A2)

The rat monoclonal antibody clone 17A2 binds to the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma, and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR) and the CD3 zeta chain to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in several cellular responses including, proliferation, differentiation, production of cytokines, or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. PRODUCT DETAILSClone: 17A2Specificity: CD3Reactivity: MouseFormat: cFluor® B532Isotype: Rat IgG2b, kTested Dilution: 5 µl/TestApplication: Flow CytometryStorage: 2-8°C protected from light. Do not freezeFormulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)Data Sheets: TDS | SDS RECOMMENDED USAGEEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. Please briefly centrifuge the reagent vial before use. Use appropriate personal protective equipment per the product safety data sheet when using this product. REFERENCES Fleming TJ, et al. 1993. J. Immunol. 151:2399-2408. Sasmono RT, et al. 2007. J. Leukoc. Biol. 82: 111-123. Kamaly N, et al. 2013. Proc. Natl. Acad. Sci. USA. 110: 6506-6511. Stinn W, et al. 2013. Toxicol. Sci. 131: 596-611. Enoksson M, et al. 2013. Blood. 121: 530-536. For research use only. Not intended for use in diagnostic procedures. cFluor® B532 is equivalent to CF®503R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.