UElandy Plasmid Miniprep Kit UE-MN-P-10, Rapidly purify up to 20 μg of plasmid DNA

The UElandy Plasmid Miniprep Kit uses the principle of SDS-alkali lysis of bacterial cells and silica gel membrane-specific binding of plasmid DNA to rapidly prepare up to 20 ug of plasmid DNA. Purified plasmids are suitable for DNA sequencing, restriction digestion, bacterial transformation, library construction, in vitro transcription and translation, molecular library construction, and routine cell transfection. Product Features High yield: up to 20 μg of plasmid DNA Wide range of applications: both high-copy and low-copy plasmids are applicable Fast: no ethanol required, single tube preparation takes less than 20min Safety: no contamination by organic reagents such as phenol and chloroform Two purification methods are available: centrifugation and negative pressure High purity: suitable for a variety of follow-up experiments Product Applications DNA sequencing Restriction digestion Bacterial transformation Library construction In vitro transcription and translation Molecular library construction Routine cell transfection Experimental Results The kits are suitable for plasmid preparation of different size Lane 1: 3kb Lane 2: 4kb Lane 3: 4.9kb Lane 4: 5kb Lane 5: 5.9kb Lane 6: 6kb Lane 7: 8 kb Lane 8: 10kb Prep plasmids are suitable for restriction digestion Lane M: UElandy 1kb DNA Ladder Lane 1,3: UElandy Kit Prep Plasmid pET-42a(+) Lane 2,4: EcoR I digested plasmid Plasmid sequencing profiles (Big Dye Terminator Chemistry, ABI 3730XL sequencer) Flow Chart