BL21 (DE3) pLysS Chemically Competent Cell

Features • Transformation efficiency: >107 cfu/μg (pUC19 DNA). • CamR. • Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without disturbing IPTG functioning. • Suitable for non-toxic and toxic protein’s expression. • Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa. Storage At -70°C for six months. Do not store in liquid nitrogen. Papers cited the use of this product: Literature Journal IF Author Date Link A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell Biosensors and Bioelectronics 5.437 Chang Zhang, et al. Lab of Biosystems and Microana 2014 Apr The original link A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11 J Virol. 4.439 Yuejun Shi, et al. 2016 Feb The original link Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor Appl Microbiol Biotechnol 3.689 Chang Zhang, et al. Laboratory of Biosystems and M 2013 Jul The original link Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxin Toxicon 2.492 Wenwu Yao, et al. 2015 Apr The original link