Multi-Layer Flask

Multi-Layer Flask USP Class VI Breathable or Sealed Cap 1 / 2 / 5 / 10 / 40 layers 656 – 25,295 cm² growth area 200 – 8,000 mL working volume TC treated / Non-treated Gamma sterilized DNase/RNase free Designed for industrial-scale adherent and suspension cell culture in biopharmaceutical manufacturing, vaccine production, and monoclonal antibody development. Hawach multi-layer flasks (also known as multi-layered cell culture flasks) are available in 1, 2, 5, 10, and 40 layers. Manufactured from medical-grade USP Class VI polystyrene in a Class 10,000 cleanroom, gamma sterilized, and certified non-pyrogenic, DNase/RNase free. Ultrasonic welding and bonding technology ensures robust, leak-free construction while saving time and space. Product Specifications – Multi-Layer Flasks TC Treated – For Adherent Cell Culture Cat. No. Layers Growth Area (cm²) Working Volume (mL) Cap Type Treatment Packaging 16051101 1 656 200 2 openings (sealed/breathable option) TC treated 4 pcs/carton 16051102 2 1296 400 2 openings TC treated 4 pcs/carton 16051103 5 3216 1000 2 openings TC treated 4 pcs/carton 16051104 10 6416 2000 2 openings TC treated 2 pcs/carton 16051105 10 6416 2000 2 openings TC treated 6 pcs/carton 16051106 40 25295 8000 2 openings TC treated 2 pcs/carton Non-Treated – For Suspension Culture or Specialized Applications Cat. No. Layers Growth Area (cm²) Working Volume (mL) Cap Type Treatment Packaging 16051201 1 656 200 2 openings Non-treated 4 pcs/carton 16051202 2 1296 400 2 openings Non-treated 4 pcs/carton 16051203 5 3216 1000 2 openings Non-treated 4 pcs/carton 16051204 10 6416 2000 2 openings Non-treated 2 pcs/carton 16051205 10 6416 2000 2 openings Non-treated 6 pcs/carton 16051206 40 25295 8000 2 openings Non-treated 2 pcs/carton Note: 10-layer flasks are offered in two pack sizes (2 or 6 units per carton) to accommodate different production scales. All flasks feature two openings for filling, sampling, and gas exchange. How to Choose the Right Multi-Layer Flask – Selection Guide Layer Count – Scale Your Culture Layers Growth Area Working Volume Typical Application 1 layer 656 cm² 200 mL Lab-scale process development, small batches 2 layers 1296 cm² 400 mL Pilot runs, scale-up studies 5 layers 3216 cm² 1000 mL Medium-scale production 10 layers 6416 cm² 2000 mL Large-scale manufacturing, vaccine production 40 layers 25295 cm² 8000 mL Industrial mass production, monoclonal antibodies TC Treated vs Non-Treated TC Treated (Tissue Culture treated): Surface modified by plasma or corona discharge to enhance hydrophilicity and cell attachment. Recommended for adherent cells (e.g., CHO, HEK293, MSCs, fibroblasts). Non-Treated: Original hydrophobic surface. Recommended for suspension cells (e.g., hybridomas, lymphocytes, insect cells) or when cells do not require attachment. Cap Options – Sealed vs Breathable Sealed cap: Provides complete closure – suitable for closed system processing or when gas exchange is not critical. Breathable cap (with hydrophobic membrane): Allows sterile gas exchange (CO₂/O₂) while preventing contamination and liquid spillage. Recommended for most cell culture applications requiring incubation with 5-10% CO₂. Please specify cap preference when ordering. Applications – Large-Scale Cell Culture with Multi-Layer Flasks Monoclonal Antibody Production Ideal for CHO and hybridoma cell culture at 10-40 layer scale. High surface area maximizes antibody yield in a compact footprint. Vaccine Manufacturing Adherent Vero, MDCK, or MRC-5 cells for viral vaccine production. TC treated 5-40 layer flasks support high-density culture. Cell & Gene Therapy Scale-up of adherent stem cells (MSCs) or viral vector production in 1-10 layer flasks under GMP-like conditions. Recombinant Protein & Biosimilar Development Large-scale expression of recombinant proteins using HEK293 or CHO cells. 10-layer and 40-layer formats reduce handling steps. Single-Layer Flask vs Multi-Layer Flask vs Cell Factory Choose the Right Cell Culture Vessel for Your Scale Why Choose Hawach Multi-Layer Flasks? USP Class VI Medical Grade High-molecular polystyrene – biocompatible, low leachables, suitable for GMP processes. Class 10,000 Cleanroom Manufactured in ISO 7 (Class 10,000) environment – ensures low particulate and consistent quality. Ultrasonic Welding + Bonding Robust, leak-free construction eliminates solvents or adhesives that could leach into culture. Gamma Sterilized Sterility assurance level (SAL) 10⁻⁶. Ready-to-use, non-pyrogenic, DNase/RNase free. TC or Non-TC Options One product line covers both adherent and suspension workflows. Breathable Cap with Hydrophobic Membrane Sterile gas exchange, prevents contamination – ideal for CO₂ incubators. Technical Specifications – Multi-Layer Flask Parameter Value Material Polystyrene (PS), USP Class VI medical grade Layers available 1, 2, 5, 10, 40 Growth area per layer approx. 632-656 cm² (depending on layer count) Total growth area 656 – 25,295 cm² Working volume per layer 200 mL (1-layer) to 200 mL per layer (10-layer = 2000 mL, 40-layer = 8000 mL) Manufacturing environment Class 10,000 (ISO 7) cleanroom Sterilization Gamma irradiation (SAL 10⁻⁶) Pyrogen Non-pyrogenic (endotoxin <0.5 EU/mL) DNase / RNase Free Cap options Sealed cap or breathable cap with hydrophobic membrane Ports 2 openings (filling and sampling/venting) Compatibility & Workflow Integration Hawach multi-layer flasks are designed to fit standard cell culture incubators, biosafety cabinets, and automated handling systems. The compact footprint (same external dimensions for 1-10 layer units) allows easy scale-up without changing equipment. Breathable caps ensure compatibility with humidified CO₂ incubators (5-10% CO₂, 37°C). For GMP manufacturing, we provide batch-specific certificates of analysis, irradiation certificates, and sterility test reports. Frequently Asked Questions What is the difference between TC treated and non-treated multi-layer flasks? TC treated flasks have a hydrophilic surface modified for optimal cell attachment – essential for adherent cells (e.g., CHO, Vero, MSCs). Non-treated flasks retain the original hydrophobic surface and are suitable for suspension cells (e.g., hybridomas, lymphocytes) or applications where attachment is not required. What is the minimum order quantity (MOQ)? MOQ is 1 carton (pack sizes vary: 2, 4, or 6 units per carton). Sample units (1-2 flasks) are available for evaluation. Can I get free samples? Yes – we offer limited sample units for process validation. Shipping fee may apply. Are these flasks compatible with robotic liquid handlers? Yes – the standard footprint and dual-opening design allow automated filling, sampling, and harvesting. We recommend compatibility testing with your specific system. What cap options are available? Both sealed caps and breathable caps (with hydrophobic membrane for sterile gas exchange) are available. Please specify your preference when ordering. Can I use these flasks for suspension cells? Yes – choose non-treated version for suspension cells. For high-density suspension, consider our breathable cap option to maintain proper gas exchange. How do I know which layer count to choose? Start with 1-layer for process development, then scale vertically using the same footprint. 5-10 layers for pilot batches, 40 layers for full-scale production. Contact our application specialists for scale-up guidance. Can I get OEM labeling or custom packaging? Yes – private label, custom barcoding, and non-standard pack sizes are available for volume orders. MOQ for OEM: 50 units per SKU. Trust & Support – Why Hawach Class 10,000 (ISO 7) cleanroom -no cross-contamination risk USP Class VI polystyrene -biocompatible for pharmaceutical use Gamma irradiation validated -SAL 10⁻⁶ Global supply chain -stock in US, Europe, Asia Technical support for scale-up, process optimization, and regulatory documentation Contact Hawach for a Quotation Enter your email address and our sales staff will contact you as soon as possible. Your Email (required) Your Message (required) Δ document.getElementById( "ak_js_8" ).setAttribute( "value", ( new Date() ).getTime() ); ×