cFluor^® UV395 Anti-Human CD45RA (HI100)

The mouse monoclonal antibody clone HI100 binds to the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions by regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO, and others. PRODUCT DETAILSClone: HI100Specificity: CD45RAReactivity: HumanFormat: cFluor® UV395Isotype: Mouse IgG2b, kTested Dilution: 5 µL/TestApplication: Flow CytometryStorage: 2-8°C protected from light. Do not freezeFormulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)Data Sheets: TDS | SDS RECOMMENDED USAGEEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. Please briefly centrifuge the reagent vial before use. Use appropriate personal protective equipment per the product safety data sheet when using this product. For research use only. Not intended for use in diagnostic procedures. Cytek® cFluor® UV395 is StarBright™ UltraViolet 400 Dye, manufactured and provided by Bio-Rad, Inc.StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.