Collagenase II _ 40508ES

Collagenase is a protease (endopeptidase) that specifically recognizes the Pro‑X‑Gly‑Pro motif — abundant in collagen and uncommon in other proteins — and cleaves the peptide bond between residue X and glycine (Gly). While many proteases digest single‑stranded, denatured collagen peptides, collagenase is unique in its ability to degrade native collagen fibers with an intact triple‑helical structure, the major structural component of connective tissues. This product is isolated from *Clostridium histolyticum* and supplied as a crude enzyme blend. It contains collagenase (formally clostridiopeptidase A), which digests native collagen and reticular fibers, alongside auxiliary proteases, polysaccharidases and lipases. These accessory enzymes break down proteins, polysaccharides and lipids within the extracellular matrix of connective and epithelial tissues, making this blend well suited for tissue dissociation. Commercially available bacterial collagenases are classified into four types—Type I, II, III, and IV—primarily based on differences in enzymatic activity profiles, each with distinct application preferences: Type I Collagenase: Exhibits a balanced profile of collagenase, caseinase, clostripain and trypsin‑like activity. Widely used for isolating epithelial cells and cells from liver, lung, adipose and adrenal tissue. Type II Collagenase: Exhibits higher clostripain activity. Typically used for preparing cells from heart, bone, skeletal muscle, thymus, and cartilage. Type III Collagenase: Features lower overall protease activity. Often used for mammary cell isolation. Type IV Collagenase: Contains low trypsin-like activity. Preferred for pancreatic islet cell isolation or any application requiring preservation of cell surface receptor integrity. This product is Type II Collagenase with a specific activity of ≥125 U/mg solid. It is suitable for dissociating tissues and cells from heart, thyroid, salivary glands, liver, bone and cartilage. Enzyme Product Selection Guide Product Form Cat. No. Product Name Source Advantages Application Liquid; ready to use 40134ES PerfCell Tryzyme™ Express, with Phenol Red Animal-component free Gentle dissociation; preserves cell viability; stable at room temperature for convenient use Primary cells requiring intact cell-surface proteins, etc. 40135ES PerfCell Tryzyme™ Express, Phenol Red-free 40514ES Recombinant Trypsin Digestion Solution, Phenol Red-free 40506ES ACCUTASE Cell Dissociation Solution Trypsin substitute; gentle dissociation; no washing or neutralization required Most primary cells, cell lines, and insect cells; ideal for stem cells, surface markers, flow cytometry, and bioreactors Powder; flexible preparation; high stability 20416ES Recombinant Trypsin (Mass Spectrometry Grade) Pichia pastoris High-purity β-trypsin >80%; activity >6800 USP units/mg; no viral contamination or chymotrypsin; ~50% lower missed-cleavage rate Continuous culture of adherent cells; protein mass spectrometry, sequencing, and peptide mapping 40512ES Recombinant Trypsin (Pharmacopoeia Grade) E. coli High-purity β-trypsin ≥70%; activity >3800 USP units/mg; no viral contamination or chymotrypsin 40507ES Collagenase I Clostridium histolyticum Contains a relatively balanced spectrum of activities (including collagenase, clostripain, clostridiopeptidase, and trypsin) Epithelial, liver, lung, adipose, and adrenal tissues 40508ES Collagenase II Clostridium histolyticum Contains higher clostripain activity Heart, bone, muscle, thymus, and cartilage 40509ES Collagenase III Clostridium histolyticum Contains lower protease activity Mammary cell and fetal cell preparation 40510ES Collagenase IV Clostridium histolyticum Contains low trypsin activity slet cells, stem cells, and applications requiring receptor integrity 40511ES Collagenase V Clostridium histolyticum Higher collagenase/clostripain activity; lower trypsin to protect membrane proteins Pancreatic islet cells and connective tissue cells 40104ES Dispase II Bacterial Fast, gentle, minimal cell damage; maintains membrane integrity; animal-virus free Tissues, primary cells, and organoids 60373ES Papain from Papaya Latex Papaya latex High specific activity (≥15 units/mg); broad substrate range; mild conditions; room-temperature stable Primary/neuronal cells (e.g., cortical neurons, smooth muscle); Fab fragment preparation; membrane protein solubilization [Note]: Phenol red-free trypsin solutions are more suitable for: flow cytometry (to avoid phenol red interference with fluorescence signals); hormone-related research (e.g., experiments with estrogen-sensitive cells such as breast cancer cells and endometrial cells); serum-free or special culture systems (where phenol red may interfere with sodium-potassium balance or medium components). Components Components No. Name 40508ES60 40508ES76 40508 Collagenase II 100 mg 1 g Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes collagen to yield amino acids equivalent to 1 µmol of L-leucine within 5 hours at 37℃ and pH 7.5.