250 mL Centrifuge Bottles – 6,000 ×g, Sterilized, Non-Pyrogenic

250 mL Centrifuge Bottles – 6,000 ×g, Sterilized, Non-Pyrogenic Standard Large-Volume Centrifuge Bottle Compatible with Nalgene, Thermo & Corning Rotors. Hawach 250 mL centrifuge bottles are designed for large-volume centrifugation, bulk biological sample processing, cell harvesting, microbial precipitation and routine bioprocessing workflows. Manufactured from high-purity virgin polypropylene in standardized cleanroom environments, these bottles deliver leak-resistant sealing, reinforced wall stability and consistent non-pyrogenic grade performance. Validated up to 6,000 ×g under standard rotor-supported conditions, the bottles match mainstream large-volume rotor dimensions, requiring no adapters or workflow adjustments. Quick Technical Summary Item Specification Product Type 250 mL large-volume centrifuge bottle Bottle Style Standard conical design Maximum Validated RCF 6,000 ×g Material High-purity virgin polypropylene Cap Type Leak-resistant screw cap Sterility Grade Factory sterilized Biological Grade Non-Pyrogenic Temperature Resistance -80°C to +121°C Typical Applications Cell harvesting, microbial collection, liquid separation, bioprocessing Decision Table Problem Solution in Hawach Bottle Outcome Impact Fluid leakage at 6,000 ×g Leak-resistant screw cap, optimized thread seal Minimized sample loss, zero rotor contamination High (critical for bioprocessing safety) Bottle deformation under full load Reinforced integrated wall structure Stable shape, consistent pelleting across batches High (cost saving, reduced reruns) Pyrogen residue interfering with cell culture Non-pyrogenic manufacturing & screening Reliable cell assay data, no pyrogen-related inhibition High (regulatory compliance) Temperature cycling failure (freeze/autoclave) -80°C to 121°C validated tolerance One bottle handles storage, sterilization, and centrifugation Medium (workflow simplification) Chemical incompatibility (solvent swelling) High-purity PP, broad chemical resistance No material degradation, safe with alcohols & weak acids Medium (operational reliability) Common Problems in Large-Volume Centrifugation Laboratories running large-volume workflows often encounter: Bottle leakage during medium-speed high-load runs Structural deformation under repeated 6,000 ×g cycles Pyrogen residue interfering with cell culture assays Sample degradation due to improper temperature tolerance Chemical incompatibility causing material swelling Batch inconsistency affecting reproducibility Hawach 250 mL centrifuge bottles are engineered to mitigate all these risks. Rotor Compatibility Matrix Rotor Type Compatibility Swing Bucket Rotor Fully compatible Fixed Angle Rotor Fully compatible 250 mL Standard Cavities Dimension match Major brand rotor compatibility – Beckman, Thermo Scientific, Eppendorf, Sorvall, Hettich, VWR. No adapter or rotor parameter adjustment required. Compatible Replacement For Hawach 250 mL centrifuge bottles adopt industry-standard dimensional and structural design, serving as an equivalent, rotor-compatible replacement for: Nalgene large-volume centrifuge bottles Thermo Scientific 250 mL centrifuge bottles Corning large-volume centrifuge containers Seamless switching – no workflow modification needed. Temperature Resistance Performance Temperature Condition Performance Ultra-low freezer (-80°C) Stable, no cracking Standard autoclaving (121°C) Compatible, 20 min cycles Freeze-thaw cycling Supported for routine lab use Recommended operating range -20°C to 50°C (optimal) Chemical Compatibility Profile Compatible With Aqueous laboratory buffers (PBS, Tris, etc.) Biological cell culture media & supernatants Alcohols (ethanol, methanol, isopropanol) Weak acids and weak bases (acetic acid, ammonia) Fermentation and microbial liquids Recommended To Avoid Strong oxidizing solvents (concentrated H₂SO₄, HNO₃) Aromatic hydrocarbons (benzene, toluene) Halogenated organic solvents (chloroform, dichloromethane) Performance Benchmark Test conditions follow ISO-like centrifugation guidelines for large-volume rotors (6,000 ×g, rated fill volume, room temperature, standard biological buffer). Internal validation protocol for lab-grade benchmark definition.* Performance Metric Generic Large–Volume Bottles Hawach 250 mL Bottles Leakage under rated RCF Occasional occurrence Significantly reduced Structural stability Deformation tendency under full load Stable, no bulging Biological purity Uncontrolled pyrogen level Consistent non-pyrogenic Temperature cycle tolerance Material aging after repeated autoclaving Stable physical property retention Batch consistency Variable quality Stable lot-to-lot traceable Technical Specifications Attribute Specification SKU Number 11351001 Nominal Volume 250 mL Product Name 250 mL Centrifuge Bottle Biological Grade Non-Pyrogenic Sterility Factory sterilized (E-beam) Maximum Validated RCF 6,000 ×g Raw Material High-purity virgin polypropylene Cap Design Leak-resistant screw cap Temperature Range -80°C to +121°C Rotor Fit Swing bucket / fixed angle universal Related Centrifuge Bottle Sizes Volume Max RCF Sterility Application 50 mL centrifuge tubes 12,000 ×g Sterile routine molecular biology 250 mL centrifuge bottles 6,000 ×g Sterile bioprocessing 500 mL centrifuge bottles 6,000 ×g Sterile large-volume harvesting Outcome-Driven Laboratory Applications Biotech & Cell Culture Laboratories Used for: large-volume cell supernatant separation, suspension cell harvesting, medium concentration. – Outcome: Reduced leakage and contamination risk during repeated harvesting cycles, improving cell viability data consistency. Microbiology & Fermentation Labs Used for: bacterial/yeast strain collection, fermentation liquid clarification, large-scale microbial precipitation. – Outcome: Stable structural performance across batches, minimizing workflow interruptions and reruns. Pharmaceutical & Bioprocessing Used for: raw material separation, intermediate liquid clarification, QC sample preparation. – Outcome: Consistent batch quality fits GLP/GMP laboratory management, lowering compliance risk. Research Institutes & Industrial Labs Used for: large-volume routine centrifugation, bulk sample low-temperature archiving, general bioprocess experiments. – Outcome: Reliable daily operation with less time spent on consumable failure troubleshooting. Manufacturing & Supply Stability ISO cleanroom production -standardized environment, automated injection molding Full lot traceability -each batch documented for quality audit Stable raw material supply -long-term order continuity guaranteed OEM/private label capacity -custom printing, packaging, barcode labeling Export-ready packaging -standard cartons suitable for sea/air shipment Packaging Options Catalog Number Description Packaging 11351001 250 mL Centrifuge Bottle, Sterilized, Non-Pyrogenic 4 pcs/bag, 25 bags/carton (100 pcs/carton total) OEM & Bulk Supply Support Available services: Custom logo printing (MOQ available on request) Private labeling and tailored packaging Distributor reserved stocking programs Full lot traceability documentation COA, sterility, and non-pyrogenic certificates provided Long-term fixed-price agreements for institutional and distributor partners Frequently Asked Questions Technical FAQs Are these 250 mL centrifuge bottles sterilized? Yes, factory sterilized (E-beam) and ready for direct laboratory use. What is the maximum centrifugal force rating? Validated for stable performance up to 6,000 ×g under standard rotor-supported conditions. Are the bottles non-pyrogenic? Yes, all production batches maintain consistent non-pyrogenic grade. Can I autoclave them? Are they freezer safe? Yes. Stable at -80°C long-term storage and compatible with 121°C autoclaving (20 min cycles). Which rotors and brands are compatible? Fits swing bucket and fixed angle 250 mL standard cavities. Compatible with Beckman, Thermo, Eppendorf, Sorvall, Hettich, VWR. Can it replace Nalgene, Thermo, or Corning 250 mL bottles? Yes. Standard dimensions and rotor-friendly design allow direct workflow replacement without equipment adjustment. Procurement & Distributor FAQs Do you support annual price locking for long – term contracts? Yes, for institutional labs, biopharma enterprises, and regular distributor partners. Can you provide distributor reserved inventory programs? Yes, reserved stock allocation and phased delivery schedules available. Are pallet, weight, and container loading specs available? Yes, full shipping data provided for bulk ocean shipments. Can you support custom barcode labeling and private branding? Yes, for qualified bulk and OEM orders. Do you allow split shipments or regional warehouse delivery? Flexible arrangements for long-term cooperative clients. Request Samples or Bulk Quotation Request free evaluation samples, rotor compatibility confirmation, bulk pricing, OEM customization, QA documentation, or distributor partnership details. Submit your annual consumption volume and packaging preference – you will receive a customized quotation within 24 hours.