LwaCas13a Nuclease (10 µM) _ 14709ES

LwaCas13a Nuclease is an RNA-guided RNA endonuclease derived from Leptotrichia wadei. Its activity depends on an RNA-mediated recognition mechanism. In the presence of a suitable protospacer flanking site (PFS) sequence on the target single-stranded RNA (ssRNA), LwaCas13a specifically recognizes and cleaves the target ssRNA under the guidance of a CRISPR RNA (crRNA).In addition, LwaCas13a possesses target-dependent trans-cleavage (collateral cleavage) activity. This activity is triggered only after the formation of a ternary complex consisting of LwaCas13a, crRNA, and the target ssRNA. Once activated, the enzyme not only efficiently cleaves the target RNA but also non-specifically degrades any single-stranded RNA present in the reaction system. Leveraging this signal-amplification property, LwaCas13a is widely used in the development of rapid nucleic acid detection kits and holds significant value in the field of molecular diagnostics. Specifications Source Recombinant expression in Escherichia coli of the Cas13a gene from Leptotrichia wadei Molecular weight 138.5 kDa Concentration 10 μM Purity ≥95% Tag None Storage buffer 25 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol (pH 8 at 25℃) Components Component No. Component 14709ES77 14709ES88 14709-A LwaCas13a Nuclease (10 μM) 70 μL 350 μL 14709-B 10× Cas13a Reaction Buffer 1 mL 5 × 1 mL