Fold-Assist Chemically Competent E. Coli Cell Bundle

Fold-Assist Chemically Competent E. Coli Cell Bundle Designed to support the expression of challenging recombinant proteins, the Fold-Assist™ Chaperone Competent Cell Bundle features E. coli BL21 (DE3) strains engineered to promote proper folding through co-expression of specialized molecular chaperone systems. This bundle includes five strains, each carrying a distinct Fold-Assist™ chaperone plasmid (pFA series), designed to provide complementary folding environments for difficult targets. These systems correspond to widely used chaperone configurations and include combinations of DnaK/DnaJ/GrpE (KJE), GroEL/ES, and Trigger Factor: pFA-KJE-Gro — combined DnaK/DnaJ/GrpE + GroEL/ES system pFA-KJE — DnaK/DnaJ/GrpE system pFA-Gro — GroEL/ES system pFA-GroTF — GroEL/ES + Trigger Factor system pFA-TF — Trigger Factor system Each plasmid has been engineered in a stabilized, low-burden format to support consistent maintenance and reliable co-expression alongside high-level protein production systems. Built on the trusted BL21 (DE3) background, these strains enable robust T7-driven expression while minimizing proteolysis, making them ideal for producing soluble, functional protein. Key Features: Five Fold-Assist™ chaperone systems: Screen multiple folding pathways to identify optimal expression conditions Functionally aligned with industry-standard systems: Corresponding to common KJE, GroEL/ES, and Trigger Factor-based chaperone configurations Enhanced protein solubility: Reduce inclusion body formation and increase recovery of active protein Optimized plasmid design: Engineered for stable maintenance and reduced cellular burden during co-expression Compatible with standard expression vectors: Designed to perform alongside common high-expression plasmid systems Inducible chaperone expression: Fine-tune folding support independently of target protein production Free of animal derived components and grown with animal free media. Applications: Expression of insoluble or aggregation-prone proteins Production of eukaryotic or multi-domain proteins in E. coli Structural biology and protein characterization Optimization of recombinant protein yield and activity Workflow Advantage: Each strain is pre-transformed with a chaperone plasmid, eliminating the need for sequential cloning and strain construction. This enables rapid screening of multiple folding environments using a single transformation step Kit Components: 2x 100µL of each Chaperone Competent E. Coli Cell line (10x tubes in total) 2 x 12mL Recovery Media 1 x 10µL Control Plasmid (pUC19 Control, 500 pg/µL) Storage/Handling: This product is shipped on dry ice. Chemically Competent E. coli cells should be stored at -80°C, pUC19 Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.