cFluor^® BYG667 Anti-Human CD10 (HI10a)

The mouse monoclonal antibody clone HI10a binds to human CD10, a 100 kDa neutral endopeptidase that is a member of the metalloprotease family. CD10 is also known as the common acute lymphoblastic leukemia antigen (CALLA), enkephalinase, and neprilysin. In normal cells, CD10 is expressed on early B and T lymphoid precursors, neutrophils, and various epithelia. CD10 is also expressed on several lymphoma cells, including Burkitt’s, acute lymphoblastic, and follicular germinal center lymphomas. CD10 functions as an endopeptidase, is involved in B cell development, and is a mediator of neutrophil inflammatory responses. CD10 is also recognized as a biomarker for hematopoietic and tissue stem cells, with recent studies suggesting its expression may have prognostic significance in leukemia and solid tumors. PRODUCT DETAILSClone: HI10aSpecificity: CD10Reactivity: HumanFormat: cFluor® BYG667Isotype: Mouse IgG1, kTested Dilution: 5 µL/TestApplication: Flow CytometryStorage: 2-8°C protected from light. Do not freezeFormulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)Data Sheets: TDS | SDS RECOMMENDED USAGEEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. Please briefly centrifuge the reagent vial before use. Use appropriate personal protective equipment per the product safety data sheet when using this product. REFERENCES Letarte M, Vera S, et al. J Exp Med. 1988;168(3):1247-1253. van Dongen JJM, Lhermitte L, Böttcher S, et al. Leukemia. 2012; 26(9):1908–1975. Shipp MA, Look AT. Blood. 1993; 82(4):1052–1070. LeBien TW, McCormack RT, et al. Blood. 1989;73(3):625-635. For research use only. Not intended for use in diagnostic procedures. cFluor® BYG667 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.