Recombinant Human Neurotrophin 3/NT-3 Protein, Active

Product Overview Product Name Recombinant Human Neurotrophin 3/NT-3 Protein Target Symbol NTF3 Alternative Names Neurotrophin 3, NT3, NTF3, Nerve Growth Factor 2, NGF2, HDNF Accession Number P20783 Expression System E. coli. Molecular Weight 13.8 kDa (predicted) Sequence Tyr149-Thr257 Purity Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining Activity Determined by in-house activity assay Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4 Endotoxin Level Available Upon Request Shipping Shipped at ambient temperature. Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. Reconstitution Reconstitute at 100 ug/mL in sterile PBS. Target Function Neurotrophin 3 (NT-3) belongs to the neurotrophin family and primarily functions as a growth factor that promotes the survival, growth, and differentiation of neurons and oligodendrocytes. NT-3 stimulates the growth and branching of axons and dendrites. This process is crucial for the establishment of neuronal circuits during development and for the regeneration of neural connections after injury. NT-3 enhances neuroprotection and regulates synaptic plasticity in mature neurons. Outside of the CNS, NT-3 promotes differentiation and survival of peripheral, motor, and sensory neurons. NT-3 primarily binds to TrkC as the high affinity signaling receptor, but association with TrkB and the low-affinity p75-NTR has been reported. In cell culture, NT-3 has been used to maintain neuronal cells in culture, the generation of peripheral neurons and oligodendrocyte precursors. It has also been used to differentiate human pluripotent stem cells (hPSCs) to glutamatergic and GABAergic neurons and the culture of 3-D organoids. Tissue Specificity NT-3 expression has been identified in brain (cerebral cortex, hippocampus, cerebellum), spinal cord, peripheral nerves, ganglia, and to a lesser extent in skin, skeletal muscles, heart, kidney, and liver. Cellular Localization Secreted protein Involvement In Disease Dysregulation of NT-3 is associated with Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, diabetic neuropathy, Charcot-Marie-Tooth disease, depression, anxiety, amyotrophic lateral sclerosis (ALS), and muscular dystrophies.