Live Cell Imaging & Analysis System

Product Overview CytoSight™ is an imaging and analysis system designed for medium-to-high-throughput live-cell experiments. With a compact footprint of just 48 × 48 × 33 cm, it integrates a 1/1.2″ color global-shutter CMOS sensor, long-working-distance phase-contrast objectives, brightfield/phase-contrast/fluorescence multi-channel optical paths, and a dual-tray parallel imaging mechanism. It can be placed inside a standard CO₂ incubator for true in-situ, long-term live-cell observation, or operate on a regular lab bench for high-throughput plate scanning, making it an ideal data acquisition platform for cell biology, drug screening, organoid research, and related fields. Parallel throughput: Dual-tray design, loading up to 6 standard microplates simultaneously Objective configuration: 4× / 10× / 20× long-working-distance phase-contrast objectives Optical channels: Brightfield + Phase-contrast + Fluorescence (red/green standard, expandable) Camera specifications: 1920 × 1200 global-shutter CMOS, 5.86 μm large pixels Environmental adaptability: 0–42°C, 5–95% RH non-condensing; compatible with CO₂ incubator placement Six Core Advantages Environmental Compatibility — The reliable choice for incubator operation The entire instrument can operate inside a CO₂ incubator, working stably at 0–42°C and 5–95% RH without condensation. This ensures that cells remain in their optimal growth environment from the outset, enabling true in-situ, live, long-term observation without the need for gas-line modifications or cumbersome external temperature control setups. Precise & Stable Imaging — Professional-grade optical hardware Equipped with a 1/1.2″ color CMOS global-shutter sensor at 1920 × 1200 resolution with 5.86 μm large pixels, paired with long-working-distance objectives, it delivers clear, low-noise, rolling-shutter-artifact-free cell images while maintaining a wide field of view, providing a reliable data foundation for downstream quantitative analysis. High-Throughput Parallel Imaging — Dual-tray, 6-plate simultaneous operation The innovative dual-tray architecture supports up to 6 standard microplates simultaneously, covering 6/12/24/48/96-well formats and compatible with Petri dishes, T-flasks, and counting chambers. Multiple experimental conditions can be acquired in parallel in a single run, significantly shortening drug screening and condition optimization cycles. Intelligent Automation — Unattended long-term imaging Fully automated plate scanning, auto/manual focusing, multi-field stitching, and long-term time-lapse imaging are integrated into one system. Once the protocol is set, it runs unattended for days or even weeks, continuously recording cellular dynamics — generating data through nights and weekends. Multi-Modal Optical System — One instrument, versatile and expandable Brightfield, phase-contrast, and fluorescence (red/green standard, optional add-ons) channels switch freely, covering a wide range of experimental needs from morphological observation to labeled detection. The modular optical design accommodates both current applications and future expansion. Powerful Software Ecosystem — A one-stop platform from images to insights Pipeline-based image processing, multi-dimensional quantitative analysis, statistical distributions and time-trend graphs, plate overviews, and traceable data auditing transform massive image datasets into interpretable, traceable, and publishable scientific conclusions. Intelligent Software Platform: Unlocking the Value of Data Experimental Design — Flexible and Reusable Supports independent layout for multiple containers, with the ability to assign zone names and individual schedules for cell types, initial densities, culture media, and growth conditions. Protocols can be edited and reused repeatedly, bringing clarity to complex experimental matrix management. Image Acquisition — Automated full coverage Fully automated multi-format plate scanning with per-well multi-field, multi-channel acquisition. Auto and manual focus modes are freely switchable, and long-term time-lapse imaging spans the entire experiment, ensuring no critical time point is missed. Image Processing — Streamlined pipeline workflow Multi-field auto-stitching and whole-well stitching are freely configurable. The pipeline workflow supports output in TIFF, JPG, PNG, and MP4 formats, with clear separation of raw data and processed results for easy archiving. Quantitative Analysis — Multi-dimensional cross-comparison Provides multiple measurement metrics including count, density, size, morphology, and intensity, supporting temporal growth trend analysis and cross-comparison across cell types, reagents, and conditions, helping researchers quickly identify differences and patterns. Visualization — Results at a glance Time-trend graphs, distribution histograms, plate overview maps, condition comparison charts, and other views intuitively present experimental results — generating publication- and presentation-ready figures without additional graphing tools. System Management — Compliant and traceable Built-in user permission management, system configuration, event tracking logs, and audit functions meet GLP compliance requirements, providing institutional-level assurance for standardized laboratory management and data traceability. Application Scenarios With its multi-modal optics, high-throughput parallelism, and long-term unattended operation, CytoSight™ covers the mainstream application areas in cell biology research: Tumor Cell Proliferation & Drug Screening High-throughput parallel plate acquisition combined with temporal growth curves and drug efficacy comparisons accelerates candidate compound evaluation. Stem Cell Differentiation & Colony Formation Tracking Long-term time-lapse imaging continuously records colony origins and differentiation trajectories, capturing key morphological milestones. Immune Cell Killing & Co-Culture Studies Multi-field synchronized observation of effector–target cell interactions quantifies killing kinetics. 3D Organoid & Spheroid Long-Term Observation Long-working-distance objectives accommodate 3D structures; fluorescence channels support viability and marker detection. Cell Migration — Scratch & Wound Healing Auto-stitching presents the complete scratch area, enabling quantitative analysis of migration rate and healing progression. Toxicity Testing & Cell Cycle Analysis Multi-channel fluorescence combined with morphometry simultaneously assesses cell viability and cell-cycle distribution changes. Technical Specifications Main Unit & Hardware Main Unit Dimensions (W×D×H) 48 × 48 × 33 cm Power Supply 100–240 VAC, 50/60 Hz to 24 VDC, 120W max Operating Environment 0–42°C, 5–95% RH, non-condensing Incubator Compatibility Entire instrument can be placed inside a CO₂ incubator Container Trays Dual trays, up to 2×3 = 6 microplates Microplate Formats 6 / 12 / 24 / 48 / 96 wells Extended Containers Petri dishes, T-flasks, counting chambers Imaging System Camera 1/1.2″ Color CMOS, global shutter, USB 3.1 Resolution 1920 × 1200 Pixel Size 5.86 × 5.86 μm² Imaging Channels Brightfield / Phase-contrast / Fluorescence (red & green standard, optional add-ons) Objectives 4× / 10× / 20× long-working-distance phase-contrast objectives Computer 64-bit Win10/11 Pro, ≥ 32 GB RAM, 1 TB SSD Data Storage RAID NAS expansion, 1G / 2.5Gbe Ethernet The descriptions and specifications provided herein represent baseline product information and may not reflect the latest updates. Please contact us for current specifications.