Spectrofluorometer

High Sensitivity, Bench-Friendly Spectrofluorometer at an Accessible Price Measure absorption, transmission, fluorescence emission, and fluorescence excitation Overview | Specifications | Measurements | Software | Gallery | In The Box | Technical Support A compact spectrofluorometer that ensures reliable optical measurements with consistent alignment between components. With a total footprint of less than 30cm x 30cm, the Ossila Spectrofluorometer fits on any lab bench or worktop to provide a complete stand-alone fluorescence system that includes: A hyperspectral 380 nm – 1000 nm white LED A high-power 365 nm UV light source 2 silicon photomultiplier detectors 2 ultra-fast monochromators A sample chamber with integrated filter holders Sample holders for both solutions and thin films The two integrated light sources support different measurements. The hyperspectral white LED is well-suited for absorption, transmission, and fluorescence excitation measurements, while the UV light provides excitation light for fluorescence emission measurements. Both sources have adjustable output power, short warm-up times, and longer lifetimes than traditional fluorescence spectroscopy light sources. On the detection side, the two integrated single-pixel silicon photomultipliers (SiPMs) with active temperature correction are more sensitive than standard photodiodes and offer a wide dynamic range. The sample holder can be rotated in indexed 22.5-degree increments. The integrated filter holders, meanwhile, allow bandpass filters to be easily inserted into the beam path. The system is designed as a low cost option for small labs that are otherwised priced out of calibrated, purpose-built systems. With the Ossila Spectrofluorometer, you can get reliable spectroscopy data at an affordable and upfront price, without blowing your research budget or compromising on performance with a DIY set up. Compact, Integrated System Less than 30 cm x 30 cm with all components included Intuitive Software Support for multiple measurement types and periodic measurements Highly Sensitive Detectors Silicon photomultipliers provide hassle-free low-light performance Wide Measurement Range Two integrated light sources allow you measure UV-IR with a single device Specifications Spectral Range (Broadband) 380 – 1000 nm Wavelength Accuracy <1 nm (in calibrated range) Wavelength Repeatability <0.1 nm UV Source Wavelength 365±15 nm Spectral Resolution Slit width dependant Slit Type Manual – micrometer driven with 10 μm resolution Slit Size 50 μm – 6 mm (W) x 10 mm (H) Maximum Sample Size 75 (W) x 50 (H) x 5 (D) mm Cuvette Size 10 x 10 mm; maximum height 52 mm Dimensions 280 x 260 x 100 mm Weight 4.2 kg Spectrofluorometers vs. Spectrophotometers Spectrofluorometers are similar to spectrophotometers, and often the terms are used interchangeably. They use the same components, and they build a spectrum in the same way. However, there are a few key differences between spectrofluorometers and spectrophotometers. Spectrofluorometers Spectrophotometers Number of Monochromators 2 1 Number of Detectors 2 1 Broadband Light Source UV Light Source Suitable for Absorbance Measurements? Suitable for Fluorescence Measurements? Emission and excitation spectra Spectrofluorometer Measurements The Ossila spectrofluorometer works by combining high quality components in various configurations, with the ability to select both the excitation and detection wavelength, enabling different measurements. For fluorescence measurements, a single wavelength light source is directed towards your sample to excite any fluorophores. This can use the UV light source or combine the broadband light source and excitation monochromator to create a variable light source. Which source is best depends on the measurement you are taking. Any emitted light from the sample enters the detection monochromator. This monochromator either selects a specific emission wavelength, or scans through various wavelengths before intensity is measured by the detector. For absorbance measurements, the broadband light source travels through the excitation monochromator, which again selects a specific wavelength or scans through a wavelength range. This monochromatic light is directed straight through your sample, where transmission is measured by a separate detector Setup for fluorescence measurements Setup for absorbance measurements Which Configuration Do I Need? To efficiently use a spectrofluorometer, ensure that you are using the correct combination of components. Excitation Fluorescence Measurements Emission Fluorescence Measurements Absorbance/Transmission Measurements Light Source Required Broadband light source Single wavelength light source Broadband light source Number of Monochromators Two required: Between light source and sample for wavelength scanning Between sample and detector for emission wavelength selection One required, between the sample and detector One required, between the light source and sample Spectrofluorometer Software The Ossila Spectrofluorometer software provides a user-friendly interface for setting measurement parameters and acquiring data, allowing for precise control of fluorescence (excitation and emission), absorbance, and transmission measurements. Control each component individually to identify experimental parameters, take preliminary measurements, and diagnose issues. The results panel makes it easy to plot, display, save, and analyze your data. For absorbance and transmission, the separate measurement tabs mean you just set your parameters and press go. For excitation fluorescence measurements, you can choose the best light source set up for you; use the UV LED or choose an individual wavelength from the tunable light source. Assign reference measurement and automatically perform necessary calculations for each measurement type. Automatically perform background calculations which are applied to your measurements. Perform periodic measurements (also known as kinetic series). Save data and settings to .csv files and keep your data save with the autosave function. Taking emission and excitation measurements with the Ossila Spectrofluorometer software Product Gallery Previous Next Standalone Spectrofluorescence System Consistent Alignment, Confident Measurements This standalone, integrated instrument is both affordable and high quality, removing the compromise on reliability that comes with building a DIY setup. With two integrated light sources, a hyperspectral white LED and a high-power UV light source, take advantage of the multiple measurement configurations to facilitate your optical measurements. Flexible Setup in One System Switch between measurements using the simple tab interface in the software, without having to reconfigure the component setup. The flexible sample chamber can accommodate both thin films and liquids with interchangeable sample holders. For additional measurement customization, adjust your sample rotation and add bandpass filters to the measurement pathway. Built for Small Spectroscopy Labs Measuring less than 30 cm x 30 cm, the Ossila Spectrofluorometer is easy to fit onto your work bench amongst your existing equipment and setup. Forget the inconvenience of sourcing all the necessary components just to stick to your budget, we have designed this system to be much more affordable than alternative systems, making it accessible to smaller research groups. In the Box The Ossila Spectrofluorometer Cuvette holder Thin film holder USB-C data cable USB drive with software & user manual 24V power supply Related Products [[collection handle="spectroscopy" limit="5"]] Technical Support [[contact]]