cFluor® BYG610 Anti-Human CD22 (HIB22)
The mouse monoclonal antibody clone HIB22 monoclonal binds to human CD22, a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. CD22 is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. PRODUCT DETAILSClone: HIB22Specificity: CD22Reactivity: HumanFormat: cFluor® BYG610Isotype: Mouse IgG1, kTested Dilution: 5 µl/TestApplication: Flow CytometryStorage: 2-8°C protected from light. Do not freezeFormulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)Data Sheets: TDS | SDS RECOMMENDED USAGEEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. Please briefly centrifuge the reagent vial before use. Use appropriate personal protective equipment per the product safety data sheet when using this product. REFERENCES Clark E. 1993. J. Immunol. 150:4715-4718. Shan D, et al. 1995. J. Immunol. 154:4466-4475. For research use only. Not intended for use in diagnostic procedures. cFluor® BYG610 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.cFluor® BYG610 is equivalent to RPE-CF®596R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.