pH-Sensitive Colorimetric RT-LAMP Kit _ 13920ES

This kit integrates a visible-light LAMP indicator into the formulation. The reaction buffer contains dATP, dCTP, dGTP, and dUTP—together with additional components essential for Bst II DNA Polymerase amplification—as well as a pH-sensitive indicator. The kit also provides glycerol-free Bst II DNA Polymerase, UDGase, and reverse transcriptase (RT enzyme), and is supplied with a lyophilization protectant, enabling direct preparation of lyophilized reaction systems on a standard freeze-dryer. The reagent delivers high amplification efficiency and sensitivity, with amplification results readable by the unaided eye: positive reactions turn orange-yellow while negative reactions remain magenta, providing strong visual contrast. Product Information Product Name Cat. No. Size RT-LAMP pH Sensitive Dye Chromogenic Version Freeze-Dryable Reagent 13920ES60 100 T 13920ES80 1,000 T 13920ES92 10,000 T Product Components Component No. Component Name 13920ES60 (100 T) 13920ES80 (1,000 T) 13920ES92 (10,000 T) 13920-A 2.5× pH Sensitive Reaction Buffer 1 mL 10 mL 100 mL 13920-B Glycerol-free RT Enzyme Mix 50 μL 500 μL 5 mL 13920-C Glycerol-free Bst Enzyme 30 μL 300 μL 3 mL 13920-D Lyophilization Protectant 500 μL 5 mL 50 mL Applications Features • One-step isothermal RNA amplification: visual colorimetric readout eliminates the need for electrophoresis or fluorescence instrumentation. • Rapid turnaround: pathogen status can be determined in as little as 30 minutes after sample loading. • Flexible workflow: a constant-temperature water bath or standard PCR thermal cycler (60–65°C) is sufficient—no specialized LAMP instrument required. • Lyophilization-ready: glycerol-free enzymes and a dedicated lyophilization protectant enable direct preparation of room-temperature-stable lyophilized kits. • High contrast endpoint: orange-yellow (positive) versus magenta (negative) for unambiguous visual interpretation, suitable for high-throughput population screening. Storage Part I — Components 13920-A and 13920-D [2.5× pH-Sensitive Reaction Buffer / Lyophilization Protectant] are shipped on dry ice and should be stored at -25 to -15°C with a shelf life of 6 months. Part II — Components 13920-B and 13920-C [Glycerol-free RT Enzyme Mix / Bst Enzyme] are shipped with ice packs and should be stored at 2 to 8°C with a shelf life of 3 months. FAQ Q: Can 13920ES be used directly without lyophilization? Yes. Lyophilization is optional; the kit may be used directly as a liquid formulation without any loss of performance. Q: The 13920ES protocol includes an 85°C inactivation step for 5 minutes. Should the tube be removed after this inactivation step, or after the 30-minute reaction at 60–65°C, for color observation? A: The tube should be removed for color observation only after the 85°C inactivation step (5 minutes) has been completed. Documents: Safety Data Sheet 13920_MSDS_HB260827 Manual