SCA TOX (Non-Clinical) CASA System

SCA® Motility and concentration for Toxicology Description Ideal for testing various drugs, chemicals and radiations for their toxicity. This system adheres to 21 CFR11 guidelines Features SCA® Motility and concentration detects the spermatozoa automatically Samples are analysed under negative phase contrast (mouse) and pseudo phase contrast (rat) Accurate count and motility analysis are carried out simultaneously Motile sperm is analysed by following the sperm trajectory and the velocity. The results, comprising all kinematic parameters for CASA, are then presented within seconds SCA® is a powerful tool, as CASA is the only objective method to analyse the total progressive and motile spermatozoa in a sample Save detailed data (all analysed parameters) of each spermatozoon into the database or export directly to Microsoft Excel Specifications Motility and concentration are analyzed in Phase contrast microscopy (an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image These phase shifts, although invisible, become visible when shown as brightness variations allowing the clear visualization of the semen sample). Or Fluorescence (The specimen is illuminated with light of a specific wavelength which is absorbed by the fluorochromes, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light) Images are captured with high frame rate digital camera Basler (25 , 50, 75 or up to 200 fps) Automatic detection of non homogeneous fields, allowing a consistent analysis There is a sort function that permits to create groups of sperm population, useful in any sperm research. Modifiable parameters: Particle area for sperm detection, Velocity Classification, VAP, Frame rate, Connectivity, Circularity, Parameter restriction, for example as elongation (to improve the cell detection) Other modifiable parameters: color mask of the path. Allows capture up to 30 fields of the sample Capture time is 1 second per field Compatible with several disposable analysis chambers like Leja® or Makler® counting chamber Parameters Analysed Counting Kinematic parameters: VCL, curvilinear velocity (m/s). Time-averaged velocity of a sperm head along its actual curvilinear path, as perceived in two dimensions in the microscope. A measure of cell vigour. VSL, straight-line (rectilinear) velocity (µm/s). Time-averaged velocity of a sperm head along the straight line between its first detected position and its last. VAP, average path velocity (µm/s). Time-averaged velocity of a sperm head along its average path. This path is computed by smoothing the curvilinear trajectory according to algorithms in the SCA Motility module. ALH, amplitude of lateral head displacement (µm). Magnitude of lateral displacement of a sperm head about its average path. It can be expressed as a maximum or an average of such displacements. LIN, linearity. The linearity of a curvilinear path, VSL/VCL. WOB, wobble. A measure of oscillation of the actual path about the average path, VAP/VCL. STR, straightness. Linearity of the average path, VSL/VAP. BCF, beat-cross frequency (Hz). The average rate at which the curvilinear path crosses the average path. Results Provided Sample (ejaculate or straws) Concentration in M/ml. Total analysed spermatozoa , Percentage in the sample, concentration in M/ml and in the sample volume of the spermatozoa classified by: Velocity: Rapid, Medium, slow and Static Classification: Rapid and progressive, Medium progressive, Non-progressive and Static or immotile Classification: Progressive, Motile and Static Hyperactive Average of the kinematic parameters presented in the sample Total of the head Area / velocity type Total of Round cells / velocity type Total and percentage of circular tracks ALH and BCF in Medium and Rapid Progressive Sperm Brochures SCA Toxicology Brochure SCA Veterinary Brochure