HiSalt T4 DNA Ligase (400 U/μL) _ 14967ES

HiSalt T4 DNA Ligase is a salt-tolerant mutant of T4 DNA ligase that catalyzes the formation of phosphodiester bonds between adjacent 5′ phosphate and 3′ hydroxyl termini on double-stranded DNA or RNA. Compared with the wild-type T4 DNA ligase, this enzyme exhibits stronger tolerance under high-salt conditions. Its catalytic activity depends on ATP as a cofactor and remains effective at salt concentrations up to 300 mM. It can ligate both blunt and cohesive ends, repair double-stranded DNA, and seal single-strand nicks in some DNA/RNA hybrid strands. Cat.No. Size Price 14967ES20 20 KU $65 14967ES60 100 KU $265 Components Components No. Name 14967ES20 14967ES60 14967-A HiSalt T4 DNA Ligase (400 U/μL) 50 μL 250 μL 14967-B 10×T4DL Reaction Buffer 1 mL 3×1 mL Specifications Source Recombinant expression in E. coli, derived from engineered HiSalt T4 DNA Ligase Concentration 400 U/μL Purity ≥95% Tag None Enzyme Storage Buffer 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (pH 7.4 @ 25°C) Shipping and Storage Ship with ice pack. Store at -25~-15°C. Valid for 2 year. Application 1. Ligation of restriction endonuclease-digested DNA fragments. 2. Ligation of linkers and adapters to the blunt ends of DNA fragments. Figure Salt Tolerance on Cohesive-End Substrates Figure 1. Salt tolerance of 14967ES T4 DNA Ligase. Relative activity was measured at increasing NaCl concentrations. 14967ES (red triangles) maintained high ligation activity up to 750 mM NaCl, while the wild-type T4 DNA ligase (black triangles) showed a marked activity decline above 100 mM NaCl. Documents: Safety Data Sheet 14967_MSDS_HB260918_EN.PDF Manuals 14967_Manual_Ver.EN20250914.pdf