AxoBind SureCapture-pY (AX001001) AxoBindTM SureCapture-pY 001 is a novel phosphotyrosine SuperBinder with superior enrichment capacity of traditional IMAC reagents. The covalently bound SH2 domain is optimized for broad capture of phosphotyrosine containing peptides under physiological conditions preserving the phosphate on each peptide. Support: Proprietary polymer paramagnetic microparticles Bead size: 30 µm Formulation: SureCapture-pY 25% suspension in 0.1M tris-HCl pH 7.4 AxoBind SureCapture pY Kits are assembled to provide all the optimized and standardized reagents to provide the best performance and highest reproducibility of phosphotyrosine enrichment studies The AxoBind SureCapture pY Kit contains all reagents needed to process a protein digest to enrich phosphotyrosine peptides. Additional items such as LC/MS grade methanol, low protein binding pipette tips, LC/MS buffer and ancillary equipment such as a benchtop centrifuge are not included but can be easily obtained from your life science supply company. AxoBind SureCapture pY Kit 10 x SureCapture-pY beads (NOTE: upon arrival, store kit at 4 °C, shelf life = 4 months) 1 x Resuspension Buffer (For dissolution of your dried cleaned protein digest) 1 x AxoBind Enhancer (For high recovery of pY peptides) 2 x AxoBind Buffer 1 1 x AxoBind Buffer 2 1 x AxoBind SPE Conditioning Buffer 1 x AxoBind Elution Buffer 1 x AxoBind SPE Wash Buffer 1 x AxoBind SPE Elution Buffer 10 x AxoBind-tips 1 x AxoBind tip adapter (For use in microcentrifuge tubes)