Human PBMC-derived Dendritic Cell Cytokine Set _ 92670ES

Dendritic cells (DCs) are the most potent antigen-presenting cells in the human body, serving as a bridge between innate and adaptive immunity. However, their extremely low abundance in vivo makes direct isolation difficult, which significantly limits DC research and applications. Nevertheless, DCs can be generated through the differentiation and induction of DC precursor cells from various tissues, such as bone marrow precursors, peripheral blood monocytes, and umbilical cord blood monocytes. For human studies, the most commonly used method is inducing DCs from human peripheral blood mononuclear cells (PBMCs), as peripheral blood is the most accessible source and yields the highest number of monocytes. This cytokine set includes recombinant human GM-CSF, IL-4, IL-6, TNF-α, and IL-1β. IL-4 inhibits the differentiation of monocytes into CD14+ macrophages, thereby inducing their differentiation into immature DCs. Without IL-4 in the culture system, monocytes will differentiate into macrophages. GM-CSF induces the differentiation of monocytes or DC precursors into DCs, while promoting DC proliferation and survival. Furthermore, GM-CSF upregulates surface antigens on DCs, enhancing their capacity for antigen capture and processing. The combined action of GM-CSF and IL-4 drives the directed differentiation of monocytes into immature DCs. The combination of TNF-α, IL-6, and IL-1β induces the complete maturation of DCs. Specifications Cat.No. 92670ES70 / 92670ES80 Size 200 mL / 1000 mL Components Component Number Component Name 92670ES70 92670ES80 92670-A Recombinant Human GM-CSF 20 μg 100 μg 92670-B Recombinant Human IL-4 10 μg(2×5 μg) 50 μg 92670-C Recombinant Human IL-6 10 μg 50 μg 92670-D Recombinant Human TNF-a 10 μg 10 μg 92670-E Recombinant Human IL-1β 10 μg 10 μg Product information Original Cat. No. Product Name Source Appearance Reference Concentration 91102ES Recombinant Human GM-CSF E.coli Lyophilized Powder 100 ng/mL 90105ES Recombinant Human IL-4 E.coli Lyophilized Powder 50 ng/mL 90107ES Recombinant Human IL-6 E.coli Lyophilized Powder 50 ng/mL 90635ES Recombinant Human TNF-a E.coli Lyophilized Powder 10 ng/mL 90101ES Recombinant Human IL-13 E.coli Lyophilized Powder 10 ng/mL Reconstitution Method Component Name Resuspension method Human GM-CSF Protein Human IL-4 Protein Human IL-6 Preotein Human TNF-a Preotein Human IL-1β Preotein Before opening the vial, centrifuge it first to bring the contents to the bottom. Res uspend with sterile water and reconstitute to a concentration of 0.1–1.0 mg/mL; f urther dilution can be performed according to subsequent experiments. For long term storage, it is recommended to add a carrier protein (0.1% BSA) to the dilutio n buffer. Aliquot into single-use amounts, freeze at -80℃, and avoid repeated fre eze-thaw cycles. Storage Conditions Store at -25 ~ -15℃. Valid for 1 year from the date of receipt. After reconstitution, store at 2 ~ 8℃ under aseptic conditions, valid for 7 days. Alternatively, after reconstitution, store at -85 ~ -65℃ under aseptic conditions, valid for 3 months. Notes 1. For your safety and health, please wear a lab coat and disposable gloves during handling. This product is for research use only. Documents: Safety Data Sheet 92670_MSDS_HB260701 Manuals: 92670_Manual_HB20260625