Trans2-Blue Chemically Competent Cell

Description Trans2-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 109 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR) and chloramphenicol (CamR). Genotype TetRΔ(mcrA)183Hte[F′{proAB lacIq lacZΔM15Tn10(TetR)AmyCamR}]Δ(mcrCB-hsdSMR-mrr)173 endA1supE44 thi-1 recA1 gyrA96 relA1 Features • High transformation efficiency (>1×109cfu/μg DNA). • Suitable for larger plasmids and recombinant products. • Reduced preference for plasmid size, suitable for library construction. • Used in Blue/White selection. Storage at -70 °C for six months Notes Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. Gentle handling is required for the entire procedure References: Literature Journal IF Author Date Link Identification of genes directly involved in shell formation and their functions in pearl oyster, Pinctada fucata PLoS One 3.5328 Fang D, et al. Institute of Marine Biotechnology, 2011 The original link Characterization of an insect heterodimeric voltage-gated sodium channel with unique alternative splicing mode comparative biochemistry and physiology b-biochemi 1.651 Jiang XZ, et al. Key Laboratory of Entomology and 2017 Jan The original link