DMT Chemically Competent Cell

DMT Chemical Competent cells have high transformation efficiency: >108 cfu/μg (pUC19 DNA), are resistant to T1 and T5 phage. The cells harbor DMT enzyme (DpnI) to achieve in vivo digestion of methylated DN and are ideal for some site-directed mutagenesis protocols. Genotype F- φ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 gyrA96relA1 tonA Features • High transformation efficiency: >108 cfu/μg (pUC19 DNA). • Resistance to T1 and T5 phage. • In vivo digestion of methylated DNA, suitable for site-directed mutagenesis. Storage at -70 °C for six months Notes Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. Gentle handling is required for the entire procedure. References: Literature Journal IF Author Date Link Characterization of EndoTT, a novel single-stranded DNA-specific endonuclease from Thermoanaerobacter tengcongensis nucleic acids research 9.202 Zhang S, et al. State Key Laboratory of Microbial 2010 Jun The original link Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis Biotechnology for Biofuels 6.4439 Nanyu Han,et al. 2017 May The original link A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates Pathogenicity Front Cell Infect Microbiol 5.2179 Bingqing Li,et al. 2017Feb The original link The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/??-Glucanase That Can Degrade Crystalline Cellulose Applied and Environmental Microbiology 3.668 Xianli Xue, et al. 2015 Mar The original link