Human Breast Organoid Cytokine Set _ 92673ES

Product information Product Name Catalog No. Specification Human Breast Organoid Cytokine Kit 92673ES10 10 μg 92673ES50 50 μg 92673ES60 100 μg Product introduction The human breast organoid culture cytokine kit contains seven key factors: Wnt3a, R-spondin-1, Noggin, EGF, FGF-10, NRG1, and bFGF. Here is a breakdown of their specific roles: Wnt3a is one of the most critical factors and serves as a key switch for maintaining stemness. As a ligand for the Wnt signaling pathway, it activates β-catenin signaling, directly driving the self-renewal and proliferation of breast stem/progenitor cells. R-spondin-1 acts as a potent enhancer of Wnt signaling. While it does not directly activate the Wnt pathway itself, it binds to the Lgr5 receptor to inhibit Wnt-suppressing proteins (RNF43/ZNRF3), thereby dramatically amplifying the signal strength of Wnt3a. Noggin functions as a differentiation inhibitor. As an antagonist of Bone Morphogenetic Proteins (BMPs), it counteracts BMP signaling, which typically induces cell differentiation (particularly toward a mesenchymal fate or halts proliferation). By inhibiting this pathway, Noggin ensures that cells remain in an undifferentiated stem cell state. EGF provides the fundamental drive for proliferation. By binding to cell surface EGFR, it activates downstream pathways such as MAPK/ERK, promoting mitosis and survival in epithelial cells. FGF-10 mimics epithelial-mesenchymal signaling. In vivo, FGF-10 is primarily secreted by mesenchymal cells to act on epithelial cells. In culture, it promotes the proliferation, migration, and branching morphogenesis of breast epithelial cells. NRG1 (Neuregulin 1) is essential for luminal cell differentiation and expansion. As a ligand for ErbB receptors (such as HER3/HER4), NRG1 signaling is crucial for the survival and proliferation of luminal epithelial cells in the breast. Prolactin activates the JAK2/STAT5 pathway, prompting organoids to express milk proteins (such as β-casein) and secrete lipid droplets, effectively simulating the functional state of the lactating breast. Product includes Component Number Component Name Quantity 92673ES10 92673ES50 92673ES60 92673-A Human Wnt3a Protein 1 10 μg 50 μg 100 μg 92673-B Human R-spondin-1 Protein 1 10 μg 50 μg 100 μg 92673-C Human Noggin Protein 1 10 μg 50 μg 100 μg 92673-D Human EGF Protein 1 10 μg 50 μg 100 μg 92673-E Human bFGF Protein 1 10 μg 50 μg 100 μg 92673-F Human FGF-10 Protein 1 10 μg 50 μg 100 μg 92673-G Human NRG1-β1Protein 1 10 μg 50 μg 100 μg 92673-H Human Prolactin Protein 1 10 μg 50 μg 100 μg Specifications Species Human Tag No Tag Expression System HEK293 Purity > 95% as determined by SDS-PAGE Endotoxin Level < 1.0 EU/μg (by LAL method) Appearance Liquid & Lyophilized Powder Product information Original Cat. No. Product Name Expression System Appearance 92294ES Human Wnt3a Protein HEK293 Liquid 92278ES Human RSPO1 Protein HEK293 Liquid 92623ES Human Noggin Protein HEK293 Liquid 92708ES Human EGF Protein E.coli Lyophilized Powder 91330ES Human bFGF Protein E.coli Lyophilized Powder 91306ES Human FGF-10 Protein E.coli Lyophilized Powder 92140ES Human NRG1-p1 Protein E.coli Lyophilized Powder 92604ES Human Prolactin Protein HEK293 Liquid Storage Conditions Store at -85℃ ~ -65℃. Shelf life is 1 year from the date of receipt. Avoid repeated freeze-thaw cycles; before opening the vial, centrifuge it first. It is recommended to aliquot the product into single-use portions for each experiment and then store at -80℃ after aliquoting. Reconstitution instructions Before opening the vial, first centrifuge it to bring the contents down to the bottom. Resuspend the material using sterile distilled water or PBS, and reconstitute it to a concentration of 0.1–1.0 mg/mL. Further dilution can be performed as needed for subsequent experiments. For long-term storage, it is recommended to add a carrier protein (0.1% BSA) to the dilution buffer. Aliquot the solution into single-use amounts, and store at -80°C, avoiding repeated freeze-thaw cycles. Precautions 1. For your safety and health, please wear a lab coat and disposable gloves during handling. 2. This product is for research use only. Documents: Safety Data Sheet 92673_MSDS_HB260707 Manuals: 92673_Manual_HB20260625