Firefly Glo Luciferase Reporter Gene Assay Kit _ 11404ES

Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it catalyzes the oxidation of luciferin to oxyluciferin. During the oxidation of luciferin, bioluminescence at a wavelength of approximately 560 nm is emitted, which can be measured using a luminometer. Figure 1. Detection Principle of Firefly Luciferase The Firefly Glo Luciferase Reporter Gene Assay Kit is a glow-type firefly luciferase reporter gene assay kit featuring high sensitivity and stable luminescence signal, which can meet the requirements of high-throughput detection of luciferase expression in mammalian cells. Compared with the flash-type Firefly Luciferase Reporter Gene Assay Kit (Cat#.11401), this product has the following advantages: 1) More stable luminescence signal, providing greater flexibility for experimental design. 2) Adopting an add-mix-read operation method, no need for auto-injectors, and no need to discard culture medium, centrifuge, or perform other steps, greatly simplifying the experimental workflow. 3) No obvious irritating odor. This kit is compatible with various commonly used cell culture media: RPMI 1640, DMEM, MEM-alpha, F12, DMEM/F12, etc., with a half-life of approximately 2 hours (22°C), meeting the needs of most high-throughput experiments. Product Information Product Name Catalog No. Size Firefly Glo Luciferase Reporter Gene Assay Kit 11404ES60 100 T 11404ES80 1,000 T Component Information Component No. Component Name 11404ES60 (100 T) 11404ES80 (1,000 T) 11404-A Firefly Glo Luciferase Assay Buffer 10 mL 100 mL 11404-B Firefly Glo Luciferase Substrate 1 vial 1 vial Product Features · Stable Signal — Long signal half-life of up to 2 h, ideal for high-throughput detection. · Simple Workflow — Simply add the sample and read the result. · Broad Medium Compatibility — Compatible with common culture media, including RPMI 1640, DMEM, MEM-α, F12, and DMEM/F12. Storage Transported on dry ice. Store the unopened kit at -20°C, valid for 1 year. The dissolved and aliquoted glow-type firefly luciferase substrate should be stored at -70°C protected from light for 1 year, or at -20°C for no more than 1 month. FAQ Q: Can luciferase detection be performed using a fluorescence microscope? A: No. An instrument with a bioluminescence module and full-wavelength detection (350-700 nm) is required, such as GloMax 20/20 (single-tube detection) or Biotex HTX multi-functional microplate reader (equipped with auto-injector). Q: Can the lysate after cell lysis be stored at -80°C? A: It can be stored at -80°C, similar to protein storage methods. Lysed samples can be stored at -80°C for up to six months, or at -20°C for one month. Q: What should I do if the fluorescence signal value is too high? A: Dilute the lysed sample, or reduce the cell incubation time after transfection experiments. 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