uMRT Direct RNA-Seq Kit

Get longer reads, detect more genes and isoforms, and experience improved modification signal-to-noise from your direct RNA-Seq. The UltraMarathonRT® (uMRT) Direct RNA-Seq Kit pairs the helicase-driven processivity of uMRT with the Oxford Nanopore Technologies® (SQK-RNA004) workflow. Why Choose This Kit Detect more genes, isoforms, and longer, more contiguous native RNA reads High processivity at low temperature preserves RNA integrity and reduces early terminations and read truncation. Dramatically improved detection of long transcripts Retains long RNAs that regularly degrade at high temperatures (e.g., >50°C). Generate Higher cDNA yields from your Sample uMRT’s optimized chemistry delivers higher cDNA yields for Direct RNA-Seq. Drop-in for ONT workflows Fits naturally into the Direct RNA-Seq protocol; no changes to downstream ligation, loading, or basecalling. Convenience & Cost-Savings One box contains everything you need to pair with ONT’s SQK-RNA004, eliminating the hassle and reducing the cost of ordering multiple reagents from different vendors. Applications RNA modification mapping Isoform and UTR profiling directly on native RNA Viral and host–pathogen transcriptomics Long noncoding RNAs and repetitive/high-GC regions UltraMarathonRT Direct RNA-Seq Kit Components: UltraMarathonRT® (20 U/μL) 2x RT Reaction Buffer dNTP Mix (10 mM each) RNA Conditioner 5x Ligation Buffer Q DNA Ligase (2,000,000 U/mL) RNase Inhibitor (40 U/μL) Quenching Buffer Nuclease-free Water Reagents Required but Not Supplied: Oxford Nanopore Technologies® (ONT) SQK-RNA004 or SQK-RNA004-XL Direct RNA Sequencing kit. ONT MinION®/GridION® RNA Flow Cell (FLO-MIN004RA) or PromethION® RNA Flow Cell (FLO-PRO004RA). RNA Purification Beads. Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851). RNA template: 500 ng - 1 μg total cellular RNA or 300 ng of poly (A)+ RNA is recommended for each reaction. Product Information: Catalog# R1008S (6 reactions), R1008M (48 reactions) Storage Conditions: For short duration of storage (< 6 month), UltraMarathonRT can be stored in a -20℃ freezer. -80℃ is recommended for long-term storage. All other kit components are stable in a -20℃ freezer for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity. For Research Use Only.