FuniCut™ KpnI _ 15015ES

The FuniCut™ series of restriction endonucleases are rapidly acting, genetically engineered enzymes capable of precisely cutting DNA within 5 to 15 minutes. They are ideal for the rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. The FuniCut™ series shares a universal digestion buffer, simplifying the reaction setup, and offers excellent enzyme activity redundancy, making it easy to handle substrate excess or challenging templates. Specifications Enzyme KpnI Recognition Site 5'-GGTAC↓C-3' 3'-C↑CATGG-5' Recommended Reaction Conditions 1× FuniCut™ Buffer; incubate at 37°C. Enzyme Activity 100 U/μL Inactivation Conditions Incubate at 80°C for 20 minutes. Components Components No. Name 15027ES60 15015-A FuniCut™ KpnI 200 μL 15015-B 10×FuniCut™ Buffer 2x 1 mL 15015-C 10×FuniCut™ Color Buffer* 2x 1 mL [Note]:*10xFuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct use of the product for gel electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel; the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment in 1% agarose gel.