HiActi™ Recombinant Human Galectin-4 Protein, His Tag _ 92309ES

Galectin-4 (Gal-4), a member of the galectin family, is a tandem-repeat-type galectin featuring two carbohydrate recognition domains (CRDs) on a single polypeptide chain, connected by a linker region. These CRDs are responsible for binding β-galactosides. Several binding partners for Gal-4 have been identified, including human blood group antigens, glycoproteins, the mucin-like membrane protein MUC1, glycosphingolipids, and sulfated cholesterol. Gal-4 is constitutively expressed in the intestine and stomach, uterine epithelial cells, vascular walls, as well as hippocampal and cortical neurons. It plays a crucial role in various biological processes, including lipid raft stabilization, apical protein transport, cell adhesion, wound healing, intestinal inflammation, and host defense. Specifications Cat.No. 92309ES08 / 92309ES20 / 92309ES60 / 92309ES76 Size 5 μg / 20 μg / 100 μg / 500 μg Components Name 92309ES08 92309ES20 92309ES60 92309ES76 HiActi™ Recombinant Human Galectin-4 Protein, His Tag 5 μg 20 μg 100 μg 500 μg Performance parameters Synonyms LGALS4, GAL4, L36LBP Species Human Expression Sequence AYVPAPGYQPTYNPTLPYYQPIPGGLNVGMSVYIQGVASEHMKRFFVNFVVGQDPGSDVAFHFNPRFDGWDKVVFNTLQGGKWGSEERKRSMPFKKGAAFELVFIVLAEHYKVVVNGNPFYEYGHRLPLQMVTHLQVDGDLQLQSINFIGGQPLRPQGPPMMPPYPGPGHCHQQLNSLPTMEGPPTFNPPVPYFGRLQGGLTARRTIIIKGYVPPTGKSFAINFKVGSSGDIALHINPRMGNGTVVRNSLLNGSWGSEEKKITHNPFGPGQFFDLSIRCGLDRFKVYANGQHLFDFAHRLSAFQRVDTLEIQGDVTLSYVQI with polyhistidine tag at the N-terminus. Accession P56470 Tag N-His Expression System E.coli Molecular Weight The protein has a calculated MW of 36.8 kDa. The protein migrates as 37 kDa under reducing condition Purity >98% as determined by SDS-PAGE. Endotoxin <0.1 EU per 1 μg of the protein by the LAL method. Formulation The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. Appearance Lyophilized Powder Biological activity Measure by its ability to chemoattract human THP1 cells using a concentration range of 0.5-2.5 µg/mL. Reconstitution Instructions 1)Briefly centrifuge the vial at 3000 rpm for 5 minutes before opening. 2)It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL, and let it stand for at least 20 minutes to ensure complete dissolution. 3)The reconstituted solution can be further diluted and aliquoted. When performing further dilution and aliquoting, a carrier protein (0.1% BSA, 10% FBS, or 5% HSA) must be added. For serum-free experimental requirements, a 5% trehalose solution can be used as an alternative carrier. Storage Store at -25 to -15°C. Valid for 1 year from receipt. After reconstitution, store at 2 to 8°C for up to 7 days, or at -85 to -65°C for up to 3 months. To avoid repeated freeze-thaw cycles, we recommend aliquoting the protein upon first use. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves, for your safety. Documents Safety Data Sheet 92309_MSDS_HB260723 Manuals: 92309_Manual_HB20260615