Amine-Modified Polystyrene Latex Beads (5.0-5.9 um, 5% w/v) – Research Use Only – Non Medical

Amine-Modified Polystyrene Latex Beads (5.0-5.9 µm, 5% w/v) – Catalog #B2025677 Amine-Modified Polystyrene Latex Beads (5.0-5.9 µm, 5% w/v) (Catalog #B2025677) are uniform, surface-functionalized 5 µm polystyrene microspheres supplied as a 5 mL 5% aqueous suspension. The primary amine groups on the surface enable easy covalent conjugation to carboxyl-containing molecules (proteins, antibodies, peptides, dyes, etc.) using standard EDC/NHS chemistry. These larger aminated beads are ideal for microscopy, flow cytometry, phagocytosis studies, immunoassays, cell labeling, and the development of custom microparticle-based diagnostic or therapeutic tools. Catalog number: B2025677 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 5 mL Molecular Weight or Concentration: 5% (w/v) suspension Supplied as: Aqueous suspension Applications: Covalent bioconjugation, immunoassay development, targeted microparticle preparation, phagocytosis studies, cell labeling, agglutination assays, and surface functionalization Storage: 2-8°C (do not freeze) Keywords: Amine-Modified Polystyrene Microspheres, Amine-Functionalized Polystyrene Latex Particles, Amine-Modified Polystyrene Beads, Amine-Functional Polystyrene Latex Beads, Amine-Modified Polystyrene Nanoparticles, Amine-Modified Polystyrene Colloids, Amine-Functionalized Polystyrene Spheres Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. Scientific Overview These 5.0–5.9 µm amine-modified polystyrene latex beads feature reactive primary amine groups on the surface, allowing straightforward covalent attachment of carboxyl-containing ligands via EDC/NHS chemistry. The larger micron-scale size makes them particularly suitable for direct microscopic visualization, flow cytometry, phagocytosis assays, and agglutination-based diagnostics. They serve as a versatile platform for creating custom antibody-coated beads, targeted drug-delivery microparticles, and diagnostic reagents. Key applications include: Covalent conjugation of antibodies, proteins, peptides, or dyes Phagocytosis and immune cell interaction studies Immunoassays and particle-based agglutination tests Cell labeling and separation techniques Development of targeted microparticles for drug delivery or diagnostics Usage & Handling Guidance Store at 2–8°C. Do not freeze. Gently vortex or pipette up and down before use to ensure uniform suspension. For conjugation, wash beads if necessary and follow standard EDC/NHS protocols. After coupling, quench unreacted groups with glycine or ethanolamine. Typical working concentration: 0.1–1% (w/v) for conjugation reactions Conjugation chemistry: EDC/NHS coupling to carboxyl-containing ligands Stability: Stable for months when stored refrigerated; protect from contamination What You Get 5 mL of Amine-Modified Polystyrene Latex Beads (5.0-5.9 µm, 5% w/v suspension) Reactive surface amine groups for easy covalent bioconjugation Uniform particle size distribution for reproducible results Convenient volume for multiple experiments and scale-up For research use only (RUO) Why Researchers Choose It High-density amine surface for efficient covalent conjugation 5 µm size ideal for microscopy, flow cytometry, and phagocytosis studies Stable polystyrene core with low non-specific binding Convenient 5% w/v suspension and 5 mL volume for practical lab use Versatile platform for custom functionalized microparticles and diagnostics Frequently Asked Questions (FAQ) What chemistry is used for conjugation? EDC/NHS coupling to carboxyl groups on proteins, antibodies, or other ligands. Can these beads be used in cell studies? Yes. The 5 µm size is well-suited for phagocytosis, cell labeling, and interaction assays. How should I store the product? Store at 2–8°C. Do not freeze. Mix gently before each use. Are the beads fluorescent? No. These are plain amine-modified polystyrene beads. Fluorescent versions are available separately. What is the particle concentration? 5% (w/v) suspension. Dilute as needed for your conjugation or assay protocol. This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals. References Banerjee A, Billey LO, Shelver WL. Uptake and toxicity of polystyrene micro/nanoplastics in gastric cells: Effects of particle size and surface functionalization. PLoS ONE. 2021 Dec 31;16(12):e0260803. Paget V, Dekali S, Kortulewski T, Grall R, Gamez C, Blazy K, Aguerre-Chariol O, Chevillard S, Braun A, Rat P, Lacroix G. Specific uptake and genotoxicity induced by polystyrene nanobeads with distinct surface chemistry on human lung epithelial cells and macrophages. PLoS ONE. 2015 Apr 15;10(4):e0123297. Jeon S, Clavadetscher J, Lee DK, Chankeshwara SV, Bradley M, Cho WS. Surface charge-dependent cellular uptake of polystyrene nanoparticles. Nanomaterials (Basel). 2018 Dec 10;8(12):1028. Zhang F, Wang Z, Wang S, Fang H, Wang D. Aquatic behavior and toxicity of polystyrene nanoplastic particles with different functional groups: Complex roles of pH, dissolved organic carbon and divalent cations. Chemosphere. 2019 Aug;228:195-203. Siiman O, Burshteyn A, Insausti ME. Covalently bound antibody on polystyrene latex beads: Formation, stability, and use in analyses of white blood cell populations. J Colloid Interface Sci. 2001 Feb 1;234(1):44-58. Ercan MT, Tuncel SA, Caner BE, Pişkin E. 99mTc-labelled monodisperse latex particles coated with amino or carboxyl groups for studies of GI function. 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