Recombinant Mouse Thrombopoietin/Tpo Protein, Active, Low Endotoxin

Product Overview Product Name Recombinant Mouse Thrombopoietin/Tpo Protein Target Symbol THPO Alternative Names Thrombopoietin, THPO, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth And Development Factor, Megakaryocyte Colony-Stimulating Factor, Thrombopoietin Nirs, C-Mpl Ligand, MPLLG, MGDF, THCYT1, CAMT2, MKCSF Accession Number P40226 Expression System CHO cell line, with no Fetal Bovine Serum (FBS) Molecular Weight 35.6 kDa (predicted) Sequence Ser22-Thr356 Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining Activity Determined by a cell proliferation assay using human MO7e cells. The ED50 for this effect is typically <0.5 ng/mL. Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4 Endotoxin Level <0.001 EU per 1 μg of the protein as determined by the LAL method Shipping Shipped at ambient temperature. Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. Reconstitution Reconstitute at 100 ug/mL in sterile PBS. Optionally, use PBS with 0.1% BSA. Target Function Thrombopoietin (Tpo) plays an important role in the proliferation and maturation of megakaryocytes and their progenitors in regulating platelet production. Tpo has a role in promoting the survival, self-renewal, and expansion of hematopoietic stem cells, along with primitive multilineage progenitor cells. This has led to further interest in applications for in vitro and ex vivo maintenance, expansion and differentiation of hematopoietic stem cells. Tpo is the ligand for c-Mpl (CD110). Tissue Specificity Tpo is primarily synthesized in the liver, and to lesser degrees in the kidneys, bone marrow and spleen. Cellular Localization Secreted protein Involvement In Disease Dysregulation of Tpo is associated with idiopathic thrombocytopenic purpura (ITP), aplastic anemia, myelodysplastic syndromes (MDS), thrombocytosis, hepatitis, cirrhosis, and chemotherapy-induced thrombocytopenia.