ISOkine® IL-4 Human, Animal-Free Recombinant Protein

Description ISOkine® Interleukin-4 (IL-4) Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 129 amino acids and an N-terminal 6 amino acid histidine tag for a total length of 135 amino acids and has a predicted molecular mass of 15.8 kDa. ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions. Summary Interleukin 4 (IL-4) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells. Source Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants. Manufacturer: ORF Genetics Country of Origin: Iceland Formulation 2xPBS pH 7.2, sterile filtered through 0.22 µm filter Purity > 95% by SDS-PAGE gel analysis. Reconstitution Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA). Stability The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. Biological Activity Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human IL-4 is assayed by measuring its dose-dependent effect on the proliferation of TF-1 cells. The ED50 for this effect using IL-4 is typically <0.4 ng/ml. Optimal concentration should be determined for specific applications and cell lines. Endotoxin Level Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method. Pyrogen Free Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction. Usage This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive. Synonyms Interleukin 4, IL4, B-cell stimulatory factor 1 (BSF-1), Lymphocyte stimulatory factor 1, UniProtKB# P05112. Documents Product Datasheet (PDS) Material Safety Data Sheet (MSDS) Certificate of Origin (CoO) Related Products ISOkine® Premium Animal-Free Growth Factors & Cytokines Cytokines & Growth Factors IL-4 Human, P. pastoris Recombinant Protein IL-4 Human, E. coli Recombinant Protein IL-4 Mouse, E. coli Recombinant Protein IL-4 Rat, E. coli Recombinant Protein Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.