HuMap (Hormone & Urinary Metabolites Assessment Profile)

Doctor’s Data, Inc. has served healthcare practitioners from its CLIA-licensed laboratory in St. Charles, Illinois, since 1972. HuMap is its comprehensive urinary steroid profile: parent hormones, their metabolites, and the enzyme activity between them. Four or five timed urine samples are collected over two days and analysed by LC-MS/MS. Serum reflects what is circulating at a given moment. Urine metabolites show how hormones are made, used and cleared — including liver conjugation and phase I/phase II preference. Urinary results are an endpoint, not tissue-active levels, so read them with history and, when the case is complex, with serum. The report is built as pathways. Estrogens are mapped through E1, E2, E3, and the 2-OH, 4-OH, and 16-OH routes, followed by the methoxy products reflecting COMT. Androgens follow DHEA through testosterone and 5α-DHT, with the androsterone/etiocholanolone split indicating 5α- versus 5β-reductase activity. Aromatase helps explain low testosterone or estrogen dominance. HuMap also reports a fuller progesterone metabolite set than a parent-hormone-only test — 17-OH- and 21-OH-progesterone, 5α- and 5β-pregnanediol, allopregnanolone and pregnanetriol — so you can see production, 5α/5β preference and neurosteroid conversion, not just a single progesterone value. Use HuMap for menopausal symptoms, PMS/PMDD, PCOS, endometriosis, HRT/BHRT monitoring, male androgen or prostate concerns, fatigue, insomnia, mood, weight change, and when hormone-clearance pathways matter. Test specifications Sample type: Urine — 4 timed collections over 2 days (dinner, bedtime, waking, waking +2 hours). Fifth sample only if the patient wakes to void. Premenopausal: mid-luteal (typically days 19–23). Men and postmenopausal women: any day. Turnaround time: 2 Weeks Key markers: E1, E2, E3; 2-OH / 4-OH / 16-OH and methoxy estrogens; DHEA-S, androstenedione, testosterone, epitestosterone, 5α-DHT, androsterone, etiocholanolone; progesterone, 17-OH-progesterone, 21-OH-progesterone, 5α- and 5β-pregnanediol, allopregnanolone, pregnanetriol; diurnal cortisol and cortisone; THF, 5α-THF, THE, metabolised cortisol; 2/4/16 hydroxylation, COMT, aromatase, 5α- vs 5β-reductase, 11β-HSD2; 8-OHdG, creatinine Sample Report Collection Instructions