Glutathione Agarose Resin

Glutathione agarose resin is an affinity chromatography medium used for the purification of recombinant proteins fused to a glutathione S-transferase (GST) tag. The resin consists of the tripeptide glutathione (γ-glutamyl-cysteinyl-glycine) covalently coupled to a cross-linked agarose bead support, typically through the sulfhydryl group of its cysteine residue via a spacer arm. GST has a natural, high-affinity binding site for glutathione, so GST-tagged fusion proteins bind selectively to the immobilized ligand while untagged host proteins are washed away. BiCell Scientific’s glutathione affinity resin uses 4% highly cross-linked agarose matrix with a mean size of 90 μm and capable of operating under 0.1 MPa (1 bar/14.5 psi). The hydrophilic nature of the matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. This resin binds 10 mg GST-tagged protein/mL resin and is suitable for use in batch mode, with gravity flow, or using a chromatography system.