Human TRIAP1 (Tp53-Regulated Inhibitor Of Apoptosis 1) - Recombinant Protein

Product Overview Product Name Recombinant Human TRIAP1 Protein Product Overview This recombinant human TRIAP1 protein includes amino acids 1-76aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. Target Uniprot Id O43715 Recommended Name TP53-regulated inhibitor of apoptosis 1 Gene Name TRIAP1 Synonyms TP53-regulated inhibitor of apoptosis 1, HSPC132, MDM35, P53CSV, WF-1 Species Human Predicted Molecular Mass 11.2 kDa Expression System E.coli Expression Range 1-76aa Tag N-6His Purity >90% Formulation Lyophilized Buffer Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose Storage Condition 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. Reconstitution Instruction Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. Applications Positive Control; Immunogen; SDS-PAGE; WB Research Area Cell Biology Target Function Involved in the modulation of the mitochondrial apoptotic pathway by ensuring the accumulation of cardiolipin (CL) in mitochondrial membranes. In vitro, the TRIAP1:PRELID1 complex mediates the transfer of phosphatidic acid (PA) between liposomes and probably functions as a PA transporter across the mitochondrion intermembrane space to provide PA for CL synthesis in the inner membrane. Likewise, the TRIAP1:PRELID3A complex mediates the transfer of phosphatidic acid (PA) between liposomes (in vitro) and probably functions as a PA transporter across the mitochondrion intermembrane space (in vivo). Mediates cell survival by inhibiting activation of caspase-9 which prevents induction of apoptosis. Subcellular Location Mitochondrion. Mitochondrion intermembrane space. Protein Family TRIAP1/MDM35 family