Basement Membrane Matrix, iPSC-Qualified

Description Feeder-free culture of human pluripotent stem cells depends on a consistent attachment surface. Variation in the coating matrix can affect colony attachment, morphology and expansion from one passage to the next. Biofargo Basement Membrane Matrix, iPSC-Qualified is a basement membrane extract prepared from mouse sarcoma tissue and supplied at a protein concentration of 8–12 mg/mL. It is intended for thin-layer coating of culture vessels for feeder-free culture and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). The matrix is liquid at 2–8°C and gels rapidly above 10°C. Its temperature-dependent gelation is reversible, so it can be thawed, aliquoted and diluted on ice before coating. Specifications Product Name Biofargo Basement Membrane Matrix, iPSC-Qualified SKU BFiPSCMat Product Type Basement Membrane Matrix for Pluripotent Stem Cell Culture Application Thin-layer coating for feeder-free hESC/iPSC culture and expansion Source Mouse sarcoma tissue Protein Concentration 8–12 mg/mL Buffer DMEM containing 10 μg/mL gentamicin Volume 5 mL Storage −80°C Shelf Life 24 months Grade Research Use Only Features iPSC-Qualified — Supplied for feeder-free culture and expansion of human iPSCs and ESCs. 8–12 mg/mL Protein Concentration — Basement membrane extract suitable for dilution to a thin-layer coating. Reversible Temperature-Dependent Gelation — Liquid at 2–8°C and gels above 10°C; prematurely gelled matrix can be returned to a liquid state at 4°C. Long Shelf Life — 24 months when stored at −80°C. Aliquot-Friendly 5 mL Format — Thaw once, aliquot by intended use, and avoid repeated freeze–thaw cycles. Instructions for Use Thaw the matrix overnight at 2–8°C. For best temperature control, place the vial on wet ice inside a 4°C refrigerator during thawing. Keep the thawed matrix on ice throughout handling. The matrix gels rapidly above 10°C. Choose the coating thickness and matrix concentration for your application. For feeder-free culture and expansion of hESCs and iPSCs, use a thin-layer coating, not a thick gel. Handling Notes Store at −80°C for long-term storage. Do not use matrix that has been kept at 4°C for more than 24 hours. After thawing, aliquot into sterile tubes according to intended use. Avoid repeated freeze–thaw cycles. If the matrix gels prematurely, incubate at 4°C for 12–24 hours to restore the liquid state. If the matrix looks uneven after thawing, pipette gently up and down until homogeneous. To remove air bubbles, centrifuge at 5,000 × g for 1 minute at 0–4°C. Follow appropriate laboratory biosafety procedures and wear suitable personal protective equipment. Applications Feeder-free hESC and iPSC culture — Thin-layer coating of plates and flasks for routine pluripotent stem cell maintenance. iPSC expansion — Attachment surface for scaling up human iPSC cultures. iPSC differentiation workflows — Coated surface for pluripotent stem cells before directed differentiation. Why Choose Biofargo Basement Membrane Matrix, iPSC-Qualified? It is supplied specifically for pluripotent stem cell work, with a defined concentration range, a 24-month shelf life and clear handling guidance. It pairs with Biofargo hESC/iPSC expansion media, InstantSelect passaging reagent and PluriCryo cryopreservation solution for a complete feeder-free iPSC workflow. FAQ What is this matrix used for?It is used as a thin-layer coating for feeder-free culture and expansion of human ESCs and iPSCs. What is the protein concentration?8–12 mg/mL. How should it be stored?Store at −80°C. Shelf life is 24 months. Do not use matrix kept at 4°C for more than 24 hours. What if the matrix gels before I use it?Incubate at 4°C for 12–24 hours to return it to a liquid state. Should I use a thin layer or a thick gel?For feeder-free hESC/iPSC culture and expansion, use a thin-layer coating rather than a thick gel. Is it for clinical use?No. For Research Use Only. Not intended for clinical use.