Built on decades of proven Doppler flow velocity technology and validated through hundreds of peer-reviewed publications, the PulSAR MD is the next evolution of the Doppler Flow Velocity System, which has been trusted by researchers for decades and has been cited in hundreds of peer-reviewed publications. The PulSAR MD combines high-frequency Doppler ultrasound, automated analysis, and integrated physiological monitoring into a single platform for cardiovascular and vascular research. Engineered for longitudinal studies in mice, rats, and other laboratory animals, the PulSAR MD System measures blood flow velocity, vessel elasticity, pulse wave velocity, perfusion, cardiac function, and vascular dynamics without requiring invasive surgical flow probes. Its unique pencil-style Doppler probes provide precise alignment with blood flow to improve measurement accuracy while minimizing angle-related errors commonly associated with conventional ultrasound systems. The platform supports a wide range of preclinical applications including cardiovascular phenotyping, peripheral vascular disease, cerebrovascular function, stroke research, renal perfusion, myocardial perfusion, pulse wave velocity, vascular stiffness, transverse aortic constriction (TAC) studies, and regional blood flow assessment. Integrated physiological inputs—including ECG, respiration, oxygen saturation (SpO₂), and pressure signals—allow synchronized data acquisition for complete cardiovascular analysis. PulSAR MD features fully automated software that rapidly calculates key hemodynamic parameters including peak and mean velocity, pulsatility index, resistivity index, vessel diameter, volume flow, cardiac output, ejection fraction, stroke volume, fractional shortening, and pulse wave velocity. Automated workflows reduce operator variability, improve reproducibility, and significantly increase throughput compared with manual Doppler analysis. Available with 10, 20, and 30 MHz high-frequency Doppler probes, micromanipulators, implantable Doppler transducers, and compatible physiological monitoring platforms, the PulSAR MD System provides a scalable solution for translational cardiovascular research. Whether studying vascular function, cardiac performance, or blood flow dynamics, PulSAR MD delivers reliable, reproducible data through a compact, easy-to-use platform designed specifically for modern preclinical laboratories.