uMRT Two-Step RT-PCR Kit

The UltraMarathonRT® Two-Step RT-PCR Kit is a next-generation solution for researchers needing sensitive and faithful RNA-to-cDNA conversion followed by amplification in RT-PCR experiments, regardless of RNA template length, structure, or GC content. The kit is designed for experiments requiring high sensitivity and precision, significantly enhancing gene detection with lengths ranging from hundreds of nucleotides to tens of thousands of nucleotides. Unlike other RT-PCR kits, the UltraMarathonRT RT-PCR Kit requires only 0.1 pg of RNA input for the amplification of RNAs > 10 kb. UltraMarathonRT is an ultra-processive reverse transcriptase enzyme with intrinsic helicase activity that actively unwinds RNA secondary and tertiary structures naturally without heat, faithfully producing full-length cDNAs from complex, long, or structured RNA templates that standard reverse transcriptases often miss. The DNA polymerase provided in this kit has high fidelity and is extremely processive in amplifying long cDNA (up to 30 kb) generated by UltraMarathonRT. This kit is perfect for researchers looking to achieve high-quality results using RT-PCR working with long, complex RNA and includes all necessary reagents. Key Applications: Gene expression analysis Full-length isoform analysis Full-length virus genome analysis Molecular cloning Long or structured RNA analysis with low input Kit Components: UltraMarathonRT 2x RT Buffer High Boost Nuclease-free Water dNTP mix (10 mM each) 2x PCR Master Mix Random primers (15mer) (10 µM) Oligo(dT)18 Primer (5 μM) Catalog# R1005S (20 rxns), R1005M (50 rxns), R1005L (250 rxns)