cFluor^® R780 Anti-Human HLA-DR (L243)

The mouse monoclonal antibody clone L243 binds to the human HLA-DR antigen, a 36 kDa alpha heavy chain, and a 27 kDa beta light chain heterodimeric cell surface glycoprotein in the transmembrane major histocompatibility complex 2 (MHC II) family. HLA-DR, also known as human leukocyte antigen DR isotype, is present on the surface of antigen-presenting cells including, B cells, dendritic cells, macrophages, monocytes, and activated T cells. MHC class II regulates the immune system by playing a critical role in binding and presenting antigen-derived peptides to peptide-MHC II-specific CD4 T cells. The L243 clone identifies HLA-DR molecules, whether peptide-loaded or unoccupied. It does not exhibit cross-reactivity with HLA-DP and HLA-DQ. Clone L243 interacts with a conformational epitope on HLA-DRα, contingent upon the proper folding of the αβ heterodimer. PRODUCT DETAILSClone: L243Specificity: HLA-DRReactivity: Human, African Green Monkey, Baboon, Chimpanzee, Cynomolgus Monkey, Dog, Common Marmoset, Rhesus Macaque, Squirrel MonkeyFormat: cFluor® R780Isotype: Mouse IgG2a, kTested Dilution: 5 µl/TestApplication: Flow CytometryStorage: 2-8°C protected from light. Do not freezeFormulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)Data Sheets: TDS | SDS RECOMMENDED USAGEEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. Please briefly centrifuge the reagent vial before use. Use appropriate personal protective equipment per the product safety data sheet when using this product. REFERENCES Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. Marsik C, et al. 2003. Shock 20:493-6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97-100. For research use only. Not intended for use in diagnostic procedures. cFluor® R780 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.