Recombinant Human PDGF-AA Protein, Active, Low Endotoxin

Product Overview Product Name Recombinant Human PDGF-AA Protein Target Symbol PDGFA Alternative Names Platelet-derived Growth Factor AA, Platelet Derived Growth Factor Subunit A, Platelet Derived Growth Factor A Chain, Platelet Derived Growth Factor Alpha Chain, Platelet Derived Growth Factor Alpha Polypeptide, PDGF Subunit A, PDGF A-Chain, PDGF1 Accession Number P04085 Expression System E. coli. Molecular Weight 14.3 kDa (monomer, predicted), 14 kDa and 29 kDa observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively Sequence Ser87-Thr211 Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining Activity Determined by a cell proliferation assay using mouse BALB/c 3T3 cells. The ED50 for this effect is typically <25 ng/mL Formulation Lyophilized from sterile Sodium Acetate and NaCl with Trehalose, without carrier protein. Endotoxin Level <0.02 EU per 1 μg of the protein as determined by the LAL method Shipping Shipped at ambient temperature. Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. Reconstitution Reconstitute at 100 ug/mL in sterile PBS. Target Function Platelet-Derived Growth Factor AA (PDGF-AA) is a protein with roles in regulating of many processes including embryonic development, angiogenesis, cell proliferation and differentiation. PDGF-BB acts on mesenchymal cells as a mitogen for connective tissue formation, particularly fibroblasts, vascular smooth muscle cells and glial cells. PDGF-AA is present in the alpha granules of platelets. As such, PDGF-AA promotes angiogenesis, chemotaxis of fibroblasts and collagen synthesis in tissue repair. Additionally, PDGF-AA supports development of kidney glomeruli, the differentiation of neural stem cells and has neuroprotective functions on mature neurons during injury. PDGF-AA binds to the PDGFR alpha (CD140a) homodimer only and activates the MAPK, PI3K, and STAT3 pathways. Tissue Specificity PDGF-AA expression has been identified in epithelial tissue, endothelial tissue, smooth muscle tissue, colon, heart, lung, brain and neurons. Cellular Localization Secreted protein Involvement In Disease Dysregulation of PDGF-AA is associated with atherosclerosis, pulmonary hypertension, glomerulonephritis, diabetic retinopathy, keloids, hypertrophic scars, cancer (glioma, sarcoma, and tumor angiogenesis).