Novel Farms Porcine Fibroblasts 100% Iberico

The NFC.SS1 cell line comprises immortal fibroblasts isolated from 100% Iberian (Ibérico) pigs sourced in Valladolid, Spain. This heritage breed is globally recognised for its distinctive lipid metabolism and tissue composition, making it a valuable model for cultivated meat, extracellular matrix (ECM) production, and metabolic research. Tissue was obtained via minimally invasive 3 mm dermal punch biopsy from neonatal animals (gluteal region), enabling isolation of high‑viability fibroblast populations while preserving tissue integrity and supporting strong proliferative capacity. Cells are adherent and exhibit characteristic fibroblast morphology with consistent proliferation across extended passaging. Cultures have been maintained beyond 50 passages with sustained growth and no observable senescence under standard conditions, suggesting spontaneous immortalization. These cells have been successfully used to generate structured cultivated pork prototypes, including pork loin formats produced on edible scaffolds, demonstrating capacity for scaffold integration, matrix deposition, and formation of cohesive three‑dimensional constructs.