4-Nitrophenyl acetate
type: Product
description: 4-Nitrophenyl acetate is a chromogenic substrate widely used in enzymology, analytical chemistry and biochemical research. Upon hydrolysis by esterases, lipases and other hydrolase enzymes, it releases the chromophore 4-nitrophenol, which can be readily monitored spectrophotometrically at 405 nm due to its strong absorbance in alkaline solution. The enzymes initially release 4-nitrophenol. The assay is then performed or stopped under alkaline conditions, converting it to the p-nitrophenoxide ion, which has a strong absorbance at 405 nm. This is why enzyme activity is almost always monitored at 405 nm. This property makes 4-nitrophenyl acetate an ideal substrate for enzyme activity assays, kinetic studies, inhibitor screening and method development. It is also used as an intermediate in organic synthesis and chemical research.
sku: N0184
additional property: Synonyms
  type: PropertyValue
  value: 4-NPA: 4-Nitrophenyl ester acetic acid, p-Acetoxynitrobenzene, p-Nitrophenyl acetate, p-NPA, NSC2633
additional property: Purity
  type: PropertyValue
  value: ≥98% (TLC)
additional property: Appearance
  type: PropertyValue
  value: Powder or crystals
offers: Offer
  price currency: EUR
  availability: https://schema.org/InStock
  item condition: https://schema.org/NewCondition
  sku: N0184

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