Hieff™ Mag Universal Total RNA Extraction Kit _ 18613ES

This kit is designed for the isolation and purification of high-quality total RNA from a wide variety of sample types, including animal tissues (heart, liver, spleen, lung, muscle, fat, brain, etc.), cells, common plants, and polysaccharide‑ and polyphenol‑rich plant samples. A unique magnetic bead system combined with a carefully optimized buffer formulation efficiently captures the released nucleic acids, yielding RNA of high purity with minimal carry‑over of genomic DNA, protein and other impurities. The purified RNA is suitable for a broad range of downstream applications such as RT-PCR, real-time PCR, microarray analysis, Northern blot, dot blot, poly(A) selection, in vitro translation, RNase protection assay, and molecular cloning. The kit is fully compatible with YEASEN automated nucleic acid extraction instruments and the Thermo Scientific KingFisher Flex 96 and comparable systems. YEASEN offers integrated solutions for high‑throughput automated nucleic acid extraction. Features Broad sample compatibility: Works with various animal and plant tissues, including liver, kidney, spleen, heart, skin, aquatic samples, leaves, roots, fruits, and seeds. Fast and simple: Extraction takes less than 1 hour per sample. Fully compatible with Yeasen 16/48/96-channel extraction instruments. High-purity RNA: Includes DNase I for complete DNA removal. Ready for RT-PCR, library prep, and other downstream applications. Components Category No. Name 18613ES48 Part I 18613-A DNase I 0.6 mL/tube × 1 Part II 18613-B Proteinase K 1.1 mL/tube × 1 Part III 18613-C Lysis Buffer AL 16 mL/bottle × 1 18613-D Lysis Buffer PL 50 mL/bottle × 1 18613-E Digestion Buffer DR 40 mL/bottle × 1 18613-F Binding Buffer RB 2 mL/bottle × 1. Add 28 mL isopropanol prior to first‑time use 18613-G Magnetic Bead Suspension 1.1 mL/tube × 1 18613-H Wash Buffer DW1 19 mL/bottle × 1. Add 19 mL absolute ethanol prior to first‑time use 18613-I Wash Buffer DW2 18 mL/bottle × 1. Add 72 mL absolute ethanol prior to first‑time use 18613-J Elution Buffer RE 5 mL/bottle × 1 Notes 1. If precipitation or turbidity appears in any reagent (common when stored at low ambient temperatures), warm to 30 °C and mix until fully dissolved. 2. Magnetic beads may carry over into the eluate. Avoid aspirating magnetic beads when transferring eluted RNA. 3. Avoid repeated freeze-thaw cycles of frozen samples, as this may compromise nucleic acid quality. 4. For your safety and health, wear a lab coat and disposable gloves when handling this product. 5. Several buffers contain guanidinium salts. Do NOT mix with oxidizing disinfectants such as sodium hypochlorite; toxic gas may form. Dispose of all kit‑related waste in accordance with local institutional regulations for biohazardous waste. 6. This product is for research use only.