Antibody-Oligo Conjugation Kit - Click Chemistry Oligonucleotide-Antibody Labeling, 5 Reactions, for CITE-seq, Immuno-qPCR & DNA-Barcoded Antibodies
Descriptions 5 CONJUGATIONS PER KIT. Five complete sets of activation reagents, plus oligo and antibody controls, support five independent 100 µg conjugations for less than the price of many single-reaction kits. FAST CLICK CHEMISTRY. Bioorthogonal click chemistry reaches >90% conjugation efficiency in 1–2 hours at room temperature, with no specialized equipment and minimal side reactions, >95% reagent purity by HPLC. BUILT FOR MODERN APPLICATIONS. Generate antibody-oligonucleotide conjugates (AOCs) for CITE-seq and single-cell multiomics, immuno-qPCR, proximity ligation, FISH probes, flow cytometry barcoding, and conjugate development. Specification Format 5 independent conjugations Scale 100 µg per conjugation Chemistry Bioorthogonal click chemistry Reaction Conditions 1–2 hours at room temperature Conjugation Efficiency >90% Oligo Requirement 5′- or 3′-terminal amine-modified oligonucleotide Controls Included Oligo and IgG controls Storage −20°C, 12 months (lyophilized) More Reactions. Lower Cost per Conjugation. Feature Biofargo Kit Typical Single-Reaction Kit Reactions per Kit 5 × 100 µg 1 × 100 µg Cost per Conjugation ~$90–100 $699 Chemistry Click chemistry (bioorthogonal) Bis-arylhydrazone chemistry Reaction Time 1–2 hours at room temperature ~4 hours Efficiency >90% >98% purity Controls Included Oligo + IgG controls — Features Five independent 100 µg conjugations per kit. Bioorthogonal click chemistry with >90% conjugation efficiency. 1–2 hour reaction at room temperature. No specialized equipment required. Minimal side reactions. Oligo and IgG controls included. Application The Oligo-Antibody Conjugation Kit is ideal for a broad range of applications, including therapeutic development for creating antibody–nucleic acid conjugates (ANCs) for targeted therapies; diagnostics for developing probes for qPCR, FISH, and sequencing applications; research tools for generating custom conjugates for imaging, flow cytometry, and protein–DNA interaction studies; and CRISPR and gene editing for conjugating antibodies to guide RNAs or other oligonucleotides for targeted delivery. FAQ How many conjugations can I run per kit? The kit includes five sets of activation reagents plus conjugation buffer and controls—sufficient for five independent conjugations. What oligo modification do I need? Amine-modified oligonucleotides (5′ or 3′ terminal amine). Is this suitable for CITE-seq / DNA-barcoded antibody panels? Yes. The click chemistry produces stable covalent antibody–oligo conjugates suitable for CITE-seq, immuno-qPCR, proximity ligation, and flow barcoding workflows. Do I need any special equipment? No. The reaction runs at room temperature in 1–2 hours with standard lab equipment. How should the kit be stored? Lyophilized reagents are stable for 12 months at −20°C. AmeriDx Oligo Antibody Conjugation Kit, antibody oligonucleotide conjugation, AOC, click chemistry, CITE-seq, immuno-qPCR For research use only. Not for diagnostic or therapeutic use.