PE-Cyanine7 Anti-Human TCR Cβ1 (JOVI.1)

The mouse monoclonal antibody clone JOVI.1 binds to a constant region of the T cell receptor beta chain, also known as T cell receptor beta constant 1 (TRBC1). It is expressed on a subset of TCRαβ T cells on the beta chain of the T Cell receptor. The immunogen used to generate the hybridoma were cells from transgenic animals expressing the human Vβ3-Cβ1 TCR chain that was cloned from the HA1.7 human T cell line. PRODUCT DETAILSClone: JOVI.1Specificity: TCR Cβ1Reactivity: HumanFormat: PE-Cyanine7Isotype: Mouse IgG2a, kTested Dilution: 5 µL/TestApplication: FCStorage: 2-8°C protected from light.Do not freezeFormulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azideData Sheets: TDS | SDS RECOMMENDED USAGEFlow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests. REFERENCES Minden, M D and T W Mak. 1986. Blood. 68:327 Davis, M M et al. 1997. Ciba Found Symp. 204:94 Maciocia, Paul M et al. 2017. Nat Med. 23:1416 For research use only. Not intended for use in diagnostic procedures. PE-Cyanine7 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.