PEmax mRNA ( m1ψ substitution )

PEmax represents a significant architectural optimization of the PE2 system. By incorporating an additional Nuclear Localization Signal (NLS) and a refined nCas9 domain, it achieves superior nuclear import and targeting efficiency. Unlike traditional CRISPR methods, PEmax enables precise genomic modifications without inducing double-stranded breaks (DSBs), thereby maximizing editing yield and broadening the scope of targetable base conversions with enhanced fidelity. Synthesized via in vitro transcription with a Cap 1 structure and poly(A) tail, it mimics mature mRNA to enhance stability, translation efficiency, and performance while minimizing immune responses. mRNA Length: 9289 nt Base Composition: N1-Me-pUTP (N1-mψ) Concentration: 1.0 mg/ mL Cap Modification: Cap 1 structure Poly A Tail: Yes Form: Liquid Buffer: 1 mM sodium citrate buffer, pH 6.4. Storage: Products can be stored at -80°C or below. We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles. Shipping: The products are shipped on dry ice and should be avoided for freeze-thaw cycles. Application: Gene editing Shipping Conditions: Dry ice